Molecular Probes sodium indicators allow spatial and temporal resolution of Na+ concentrations in the presence of physiological concentrations of other monovalent cations. These sodium indicators exhibit an increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength.
Indicators for Sodium
- Measure steady-state changes in Na+ concentration
- Track Na+ bursts in axons and presynaptic terminals
Indicators are available to estimate Na+ gradients in isolated mitochondria, to measure intracellular Na+ levels, to measure Na+ efflux in cells, and, in combination with other fluorescent indicators, to correlate changes in intracellular Na+ with Ca2+ and Mg2+ concentrations, intracellular pH, and membrane potential.
CA1 pyramidal neuron in a hippocampal slice filled with SBFI delivered from a patch pipette.
Used for spatial and temporal resolution of Na+ concentration and steady-state changes in sodium concentration, especially sodium/potassium ATPase studies. Also measures sodium bursts in axons and presynaptic terminals and axon initial segments.
| CoroNa Green, AM | SBFI, Tetraammonium Salt | SBFI, AM | |||
|---|---|---|---|---|---|
| Readout | Increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength | Spectral excitation shift used to estimate Na+ gradients in isolated mitochondria and intracellular Na+ levels | |||
| Range | Responds to a range of intracellular Na+ around 10 mM | Responds to Na+ in physiological range | |||
| Fluorophore | CoroNa Green | SBFI | |||
| Standard filter set | FITC | Requires excitation ratio to quantify Na+ levels | |||
| Ex/Em (nm) | ~492/516 | ~340 and 380/500 | |||
| Bibliography | Citations | Citations | |||
| Cell permeant or impermeant? | Impermeant | Cell permeant | Impermeant | Cell permeant | |
| Platforms | Imaging | Imaging | Imaging | Imaging | |
| Usage notes | Load by microinjection, patch pipette, or pinocytic loading agent | Load in cell media | Load by microinjection, patch pipette, or pinocytic loading agent | Load in cell media | |
| Format | 1 mg | 20 x 50 µg | 1 mg | 1 mg | 20 x 50 µg |
| Cat. No. | C36675 | C36676 | S1262 | S1263 | S1264 |
Resources
Molecular Probes Handbook
More Information
For Research Use Only. Not for use in diagnostic procedures.