Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with onstage incubator
Here we provide step-by-step instructions for setting up hypoxic conditions with the EVOS FL Auto Imaging System with Onstage Incubator.
Measuring oxidative stress and hypoxia in live cells or tissue can be very challenging. Here you will find useful tips and other educational resources to help you overcome these issues.
Oxidative stress and hypoxia features
Application note
On-demand webinar
Breathing new life into hypoxia research
In this webinar, we will discuss hypoxia in human diseases, classical methods for setting up hypoxic conditions, and novel instruments and reagents for imaging cells in hypoxic conditions.
Oxidative stress and hypoxia learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2014) | Oxidative stress measurements made easy: CellROX reagents with simple workflow for high content imaging | ArrayScan, CellInsight, CellROX, high content analysis, oxidative stress |
| Application note (2015) | Using time-lapse imaging with the EVOS FL Auto Imaging System | EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator |
| Application note (2015) | Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with Onstage Incubator | EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator, phagocytosis |
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Molecular Probes Handbook | Probes for nitric oxide research—Section 18.3 | NO, nitrate, nitric oxide (NO), oxidative stress, SNAP |
| Molecular Probes Handbook | Introduction to reactive oxygen species—Section 18.1 | oxidative stress |
| Molecular Probes Handbook | Generating and detecting reactive oxygen species—Section 18.2 | oxidative stress |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry | apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress |
| Scientific poster (2010) | Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs | Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy | apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress |
| Scientific poster (2011) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability |
| Scientific poster (2011) | Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2012) | Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability |
| Scientific poster (2013) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2013) | Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents from Molecular Probes | cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability |
| Scientific poster (2013) | Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents from Molecular Probes | cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress |
| Scientific poster (2015) | Intracellular detection of hypoxia in live cells | EVOS, fluorescence microscopy/fluorescence imaging, hypoxia, EVOS, onstage incubator |
| Scientific poster (2017) | Hypoxia measurements in live and fixed cells using fluorescence microscopy and high-content imaging | EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, live-cell imaging, onstage incubator |
| Video | Compilation of live cell imaging videos using Invitrogen fluorescent reagents This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes. | cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress |
| Video | Detection of oxidative stress with CellROX Green in U2-OS cells U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX Green Reagent (Cat. No. C10444) and 100 nM TMRM in Live Cell Imaging Solution (LCIS, Cat. No. A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core inverted microscope at 37 degrees C. Menadione was added to a final concentration of 100 uM after ten minutes of baseline acquisition. The time lapse data shows the loss of signal from TMRM in the red channel as mitochondrial function decreases, concomitant with the onset of a nuclear signal in the green channel as CellRox Green is oxidized and the reagent migrates to the nucleus to generate a fluorogenic response as the active form of the dye binds to DNA. | CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress |
| Webinar | Breathing new life into hypoxia research Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss: Overview of hypoxia in human diseases Classical methods for setting up hypoxic conditions Novel instruments and reagents for imaging cells in hypoxic conditions | EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator |
| Webinar | From the hood to the microscope; Revolutionizing cell-based imaging Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. Topics include: Choose a suitable imaging culture vessel for studying cell growth and viability using bright-field microscopy Label cells to maximize signal-to-noise for fluorescence imaging Prepare culture conditions and capture images without losing temporal data for time-lapse imaging Achieve hypoxic culture conditions by modifying gas conditions or employing cell spheroid cultures | cell health, EVOS, FLoid, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator |
For Research Use Only. Not for use in diagnostic procedures.