What is Next-Generation Sequencing (NGS)?
A general overview of the basics of NGS and the workflow from library construction to data analysis.
Whether you are new to next-generation sequencing (NGS) or want to learn about the various applications of NGS, we have the learning materials to help you understand the technology and get started quickly.
NGS is a high-throughput method achieved by sequencing clonally amplified DNA templates on a massively parallel scale. The speed, throughput, and accuracy achieved with NGS has revolutionized genetic science and enabled applications once thought of as science fiction.
The Importance of Throughput and Coverage
Learn about the different concepts of sequencing coverage and how changes in coverage can occur due to variations in genetic sequence. Understand why coverage affects variant discovery and how throughput is related to coverage.
DNA Sequencing Methods
Understand the advantages and disadvantages of general sequencing methods used with NGS, from whole genome sequencing to exome and targeted sequencing.
RNA Sequencing (RNA-Seq) Methods for NGS
RNA sequencing (RNA-Seq) leverages the advantages of NGS to detect and quantify RNA in a biological sample at a given point in time. This article goes over basic experimental considerations and the workflow for various RNA-Seq methods.
Targeted Sequencing Approaches for NGS
This article provides further details on targeted sequencing approaches. Learn how Ion AmpliSeq technology enables numerous applications with a rapid, simple, and highly multiplexed amplicon-based enrichment solution.
Additional Next-Generation Sequencing resources
For Research Use Only. Not for use in diagnostic procedures.