Thermo Scientific Zeba desalting products are widely used in various research applications that involve the separation of proteins, and other macromolecules from soluble low molecular weight substances (e.g., salts) that may adversely impact the stability of the analytes or interfere with downstream applications. Zeba Desalting products are also used in protein solution buffer exchange protocols for the generation of more appropriate buffers before subsequent applications such as western blotting, immunoprecipitation, activity assays, and mass spectrometry.
Zeba Desalting products yield exceptional desalting and protein-recovery characteristics and provide consistent performance over a wide range of sample sizes and protein concentrations. These desalting products speed up the desalting process by eliminating the time needed for samples to drop out of gravity flow columns. They offer a fast and efficient protocol for the collection and concentration of clean samples in one fraction, require no chromatography system, and permit multi-sample processing.
Benefits of Zeba desalting columns, plates, and cartridges:
- High performance—proprietary resin provides excellent protein recovery and greater than 95% contaminant removal
- Flexible—available in spin columns, filter spin plates and cartridges for a range of needs
- Fast— spin columns eliminate the need for fraction screening or waiting for protein to emerge by gravity flow
Choose the right desalting product for your experiment
 |  |  | |
|---|---|---|---|
Zeba Spin Desalting Columns | Zeba 96-well Spin Plates | Zeba Chromatography Columns | |
| Desalting method | Spin-column allows processing within 5-8 minutes | Chromatography | |
| Sample volume range | 2 μL–4 mL | 20–100 μL | 15–250 μL |
| Resin Bed volume | 75 μL–10 mL | 550 μL | 1 mL–5 mL |
| Resin MWCO* | 7 kDa, 40 kDa | 7 kDa, 40 kDa | 7 kDa |
| Automation | No | Yes | No |
| FPLC compatible | No | No | Yes |
| See all Zeba columns and plates › | |||
*Molecular Weight Cut-Off (MWCO)
Sample desalting using gel filtration chromatography
Zeba desalting products rely on gel filtration chromatography, where different molecular weight cut-off (MWCO) limits exclude molecules based on size. Compared with dialysis, this technology offers the advantages of speed, compatibility with organic and other solvents, and the ability to concentrate contaminating material down to a relatively small volume, which is useful for work with toxic/radioactive substances.
Zeba Desalting Handbook
Thermo Scientific Zeba Resin technology provides consistent performance and peace of mind. The unique, proprietary Zeba Resin enables better protein recovery, even for samples with low protein concentrations.
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For Research Use Only. Not for use in diagnostic procedures.