Reliable and storage-temperature-agnostic molecular diagnostics qPCR assays need to utilize reagents that are easily lyophilized and provide consistent performance even with non-ideal samples. TaqMan Lyo-Ready qPCR master mixes fulfill these requirements, making them highly suited to lyophilized qPCR assays using your chosen primers and probes.
TaqMan Lyo-Ready qPCR master mixes are designed for consistent performance even in demanding applications, including virus and bacteria detection. Easy to lyophilize and fast to re-hydrate, they ensure consistency before and after lyophilization even in multiplex reactions.
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TaqMan Lyo-Ready qPCR master mixes are available in several formulations. Customization is also available and can be requested by contacting your Thermo Fisher Scientific representative or emailing custom.solutions@thermofisher.com.
Which TaqMan Lyo-Ready master mix is right for your application?
| TaqMan 5X Lyo-Ready 1-Step Master Mix | TaqMan 5X Lyo-Ready 1-Step Master Mix with ROX | TaqMan 5X Lyo-Ready qPCR Master Mix | TaqMan 5X Lyo-Ready qPCR Master Mix with ROX | ||
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Target | RNA, DNA | DNA | RNA, DNA | RNA, DNA | DNA | DNA |
Concentration | 2.5X | 2.5X | 5X | 5X | 5X | 5X |
Excipient | Yes, pre-mixed | Yes, pre-mixed | None | None | None | None |
Passive reference dye | None | None | None | ROX | None | ROX |
Reverse transcriptase | Thermostable M-MLV | N/A | Thermostable M-MLV | Thermostable M-MLV | N/A | N/A |
Pre-PCR benchtop stability | 4 hr* | 4 hr* | <2 hr* | <2 hr* | <2 hr* | <2 hr* |
Multiplexing targets | 5 | 5 | 5 | 3 | 5 | 3 |
Sample SKU | C14031SMP (1.5 mL) | C14081SMP (1.5 mL) | C1403SMP (1.5 mL) | C1402SMP (1.5 mL) | C1408SMP (1.5 mL) | C1407SMP (1.5 mL) |
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*Recommend setting up plate on ice and running immediately. Stability is assay dependent. Greater stability has been observed for some assays.
TaqMan Lyo-Ready qPCR master mixes are reagents used in further manufacturing (lyophilization) and labeled “Caution: For use as a raw material in further manufacturing applications”, manufactured in an ISO 13485 certified and FDA registered facility, and optimized for exceptional lot-to-lot consistency, inhibitor tolerance, and multiplexing.