OpenArray™ technology uses a microscope slide–sized plate with 3,072 through-holes. Each plate contains 48 subarrays, each with 64 through-holes. Each through-hole is 300 μm in diameter and 300 μm deep and is treated with hydrophilic and hydrophobic coatings. Reagents are retained in the through-holes via surface tension (see Figure). One OpenArray plate can hold as many samples as can eight traditional 384-well plates.
Custom plate formats
Custom OpenArray plates for the QuantStudio™ 12K Flex system are offered in 11 formats to provide flexible solutions for configuring your plates for gene expression, miRNA, or genotyping analysis.
Plate format for gene expression & miRNA analysis | # of samples per plate | Minimum order (10 pack) | Total # of samples per minimum order | |
---|---|---|---|---|
Inventoried assays only | Inventoried, made-to-order, and/or custom assays | |||
18 (3X) assays | 48 | 1 | 1 | 480 |
56 assays | 48 | 1 | 1 | 480 |
112 assays | 24 | 1 | 2 | 240 (inv only)/ 480 (inv/custom) |
168 assays | 16 | 1 | 3 | 160 (inv only)/ 480 (inv/custom) |
224 assays | 12 | 1 | 4 | 120 (inv only)/ 480 (inv/custom) |
Note: There are two sets of minimum order requirements for the gene expression offerings. “Inventoried” refers to OpenArray configurations where all selected assays are inventoried . “Inventoried/made to order/custom” refers to configurations that contain at least one custom or made-to-order assay.
Plate format for genotyping analysis | # of samples per plate | Minimum order (10 pack) | Total # of samples per minimum order |
---|---|---|---|
12 assays | 144 | 1 | 1,440 |
26 assays | 96 | 1 | 960 |
60 assays | 48 | 2 | 960 |
120 assays | 24 | 4 | 960 |
180 assays | 16 | 6 | 960 |
240 assays | 12 | 8 | 960 |
OpenArray technology offers a very easy-to-use format. For most applications, assays are preloaded onto plates at our state-of-the-art manufacturing facility. To run the plates, simply mix your sample with master mix, load with the automated QuantStudio 12K Flex OpenArray AccuFill™ System, cycle, and image (see Figure below).
OpenArray technology helps streamline real-time PCR studies that use large numbers of samples, assays, or both, because a single QuantStudio 12K Flex OpenArray plate is equivalent to running eight traditional 384-well plates. The system can run up to 4 QuantStudio 12K Flex OpenArray plates simultaneously, allowing you to process up to 1,728 genotyping samples and 2,304 samples for gene expression, typically in an 8-hour day.
Enhance your genotyping throughput by thermal cycling offline using the Dual Flat Block GeneAmp™ PCR System 9700 and process a total of more than 5,184 genotyping samples in one day. This system offers interchangeable blocks, allowing you to switch to standard multi-well plate formats as well.
Visit the OpenArray application page to learn more of each application workflow and product.
For Research Use Only. Not for use in diagnostic procedures.