What are isoschizomers?
Isoschizomers are restriction enzymes that have the same recognition sequence and the same specificity. The prototype enzyme is generally the first to be discovered and characterized. Subsequent enzyme(s) discovered from different hosts that recognize and cut at the same target sequence are termed “isoschizomers” of that prototype. Because enzyme naming conventions reference the genus, species, and strain from which they were isolated, isoschizomers could have very different names, despite the sequence recognition similarities between them. For example, Anza 3 BcuI is an isoschizomer of SpeI. Review the table below outlining common Anza isoschizomers offered in our Anza Restriction Enzyme Cloning System.
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Prototype | Anza isoschizomer | Recognition and cutting site |
---|---|---|
SpeI | Anza 3 BcuI | A^CTAG T T GATC^A |
AgeI | Anza 7 BshTI | A^CCGG T T GGCC^A |
NdeI | Anza 9 NdeI | CA^TA TG GT AT^AC |
AvrII | Anza 15 XmaJI | C^CTAG G G GATC^C |
AscI | Anza 21 SgsI | GG^CGCG CC CC GCGC^GG |
PmeI | Anza 24 MssI | GTTT^AAAC CAAA^TTG |
SphI | G CATG^C C^GTAC G | |
EcoRV | Anza 26 Eco32I | GAT^ATC CAN^TAG |
ClaI | Anza 30 Bsu15I | AT^CG AT TA GC^TA |
MfeI | Anza 31 MunI | C^AATT G G TTAA^C |
SapI | Anza 33 LguI | GCTCTTCN1^ CGAGAAGN4^ |
DdeI | Anza 41 HpyF3I | C^TNA G G ANT^C |
SnaBI | Anza 43 Eco105I | TAC^GTA ATG^CAT |
SwaI | Anza 49 SmiI | ATTT^AAAT TAAA^TTTA |
HpaI | Anza 50 KspAI | GTT^AAC CAA^TTG |
PvuI | Anza 52 PvuII | CG AT^CG GC^AT GC |
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