A variety of flask sizes filled with phenol-red medium

T225 flask volume and surface area

Start your scale-up with a T225 flask that has the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes straight-neck or angled-neck options and surface treatment alternatives that help ensure your healthy tissue cells expand and grow in the flask of your choice.

Some useful numbers such as surface area and volumes of dissociation solutions are given below for T225 flasks.

Please note: The number of cells on a flask will vary with cell type. For this table, 6.3 x 106 HeLa cells were seeded and the confluent flask contained 30 x 106 cells.

T225 flasks have a surface area of 225 cm2
Dissociation reagentVolume of reagentVolume of growth medium
TrypLE Express enzyme22.0 mL45–68 mL
TrypLE Select (1x) enzyme22.0 mL
TrypLE Select (10x) enzyme2.2 mL diluted to 22.0mL in DPBS/1 mM EDTA
Versene reagent22.0 mL
Trypsin22.0 mL

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T225 flask: EasYFlask products

Ergonomically designed for maximal access to the growth surface, this low-profile flask optimizes incubator space and features angled walls for complete viewing under microscopes. Additional features include multiple cap options, and sterilization by gamma irradiation with non-pyrogenic certification.

EasYFlask product features

 

A BioLite surface treated flask with blue cap

Thermo Scientific BioLite T225 flask surface

Thermo Scientific BioLite cell culture treated flasks with filter cap and seal cap options suit a multitude of experimental needs while providing cost efficiency for a wide selection of applications in your cell culture routine.

Caps come in both vented and plug seal varieties.

Nunclon Delta treated T225 flasks

Composed of cell culture-treated polystyrene and surface treated with proprietary Nunclon Delta surface, these T225 flasks support optimal growth and attachment of cells to the growth surface.

Filtered caps allow for constant airflow while vent/close caps allow for sufficient gas exchange.

Explore cell performance with Nunclon Delta surfaces

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