We offer a full range of Brilliant Ultra Violet (BUV) and Brilliant Violet (BV) dyes for the ultraviolet (UV) and violet lasers and detector channels of your flow cytometer
Brilliant Ultra Violet dyes, Brilliant Violet dyes, and Super Bright dyes use polymer technology to absorb energy and emit light signal. These molecules are conjugated to antibodies used in flow cytometry experiments. By incorporating Brilliant Ultra Violet, Brilliant Violet, and Super Bright dyes in their experiments, scientists can take advantage of a broader range of instrument lasers and channels—including UV and Violet—to help reduce compensation necessary in panels with multiple conjugated antibodies. Super Bright dyes, Brilliant Ultra Violet, and Brilliant Violet dyes use similar polymer technologies.
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Contact us for free panel design with experts along with BVs, BUVs, and more
Why you should use our antibodies conjugated to polymer dyes (Brilliant Ultra Violet dyes, Brilliant Violet dyes and Super Bright dyes)?
- Comprehensive, full menu of Brilliant Ultra Violet, Brilliant Violet, and Super Bright Dyes for numerous extracellular and intracellular targets
- Enables full use of UV and Violet detector channels, excellent performance in high-parameter panels and compatibility with other fluorochromes
- Enhanced brightness and stability with reduced compensation issues, and compatible with spectral instruments
- Free expert panel design help available, or make your own panels with our intuitive panel builder
Which buffer should I use with Brilliant dyes?
Either the Super Bright Staining Buffer or Brilliant Staining Buffer will work to help resolve polymer dye-to-dye interactions.
Select Brilliant Ultra Violet Dyes and Brilliant Violet Dyes
Brilliant Ultra Violet dyes and Brilliant Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. BUVs and BVs are dyes used on flow cytometers equipped with UV (355 nm) and Violet (405 nm) lasers.
Brilliant Violet dyes
| Dye | Initial brightness (out of 5 maximum) | Laser (nm) | Excitation max (nm) | Emission max (nm) | Suggested bandpass filter |
|---|---|---|---|---|---|
| Brilliant Violet 421 | 4 | Violet (405 nm) | 407 | 423 | 450/50 |
| Brilliant Violet 480 | 3 | Violet (405 nm) | 440 | 479 | 525/40 |
| Brilliant Violet 605 | 4 | Violet (405 nm) | 407 | 605 | 610/20 |
| Brilliant Violet 650 | 2 | Violet (405 nm) | 407 | 649 | 660/20, 630 LP |
| Brilliant Violet 711 | 4 | Violet (405 nm) | 407 | 713 | 710/50, 690 LP |
| Brilliant Violet 786 | 3 | Violet (405 nm) | 407 | 786 | 780/60, 750 LP |
Brilliant Ultra Violet dyes
| Dye | Initial brightness (out of 5 maximum) | Laser (nm) | Excitation max (nm) | Emission max (nm) | Suggested bandpass filter |
|---|---|---|---|---|---|
| Brilliant Ultra Violet 395 | 3 | Ultraviolet (355 nm) | 350 | 395 | 379/28 |
| Brilliant Ultra Violet 496 | 2 | Ultraviolet (355 nm) | 350 | 496 | 515/30, 450 LP |
| Brilliant Ultra Violet 563 | 4 | Ultraviolet (355 nm) | 350 | 564 | Instruments with yellow-green (561 nm) laser: 585/15, 535 LP without yellow-green: 560/40, 535 LP |
| Brilliant Ultra Violet 615 | 3 | Ultraviolet (355 nm) | 350 | 616 | 610/20, 595 LP |
| Brilliant Ultra Violet 661 | 4 | Ultraviolet (355 nm) | 350 | 660 | 670/25, 630 LP |
| Brilliant Ultra Violet 737 | 4 | Ultraviolet (355 nm) | 350 | 737 | 740/35 |
| Brilliant Ultra Violet 805 | 3 | Ultraviolet (355 nm) | 350 | 805 | 820/60 or 780/60 |
Build experiments with Invitrogen Panel Builder
Insert BUVs, BVs and Super Bight dyes into experiments for analysis on instruments with spectral or conventional instruments.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our dyes and labeling reagents
Key features:
- Recommended dyes for your flow cytometer based on lasers and detection channels settings
- Dye recommendation for UV, Violet, Blue, Green, Yellow, Red and Near IR lasers
- Checkout our specials-Brilliant Ultra Violet™, Brilliant Violet™, and NovaFluor™ dyes; more dyes for spectral needs
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BRILLIANT VIOLET and BRILLIANT ULTRA VIOLET are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.