Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays
This article features an overview of the current antibodies available to act as immune checkpoints for T cell defense against invading tumors.
Find protocols, educational webinars, scientific posters and more to help you design your immunohistochemistry experiments.
Immunohistochemistry features
BioProbes article
Scientific poster
Incorporating histological analysis into existing high-content imaging
To address the need for quantitative colorimetric imaging, we have developed a method for analyzing histological samples on a high-content imaging instrument using a specialized brightfield unit
Immunohistochemistry learning resources
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| Type | Title | Categories |
|---|---|---|
| BioProbes articles (Issues 50–present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Pierce Protein Methods | Overview of immunohistochemistry (IHC) | antibodies, fixation, immunohistochemistry (IHC) |
| Pierce Protein Methods | Immunohistochemistry methods articles | antibodies, fixation, immunohistochemistry (IHC) |
| Protocol | Immunohistochemical staining of formalin-fixed paraffin-embedded tissues | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Indirect method (purified) | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Indirect method (biotin) | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE Tissue Trypsin Digestion—Direct Method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue trypsin digestion antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue low pH antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue low pH antigen retrieval—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue high pH antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue high pH antigen retrieval—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Scientific poster (2015) | Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Scientific poster (2016) | Correlating internalization and potency to accelerate antibody discovery and development | brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC) |
| Scientific poster (2016) | Incorporating histological analysis into existing high content imaging | antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC) |
| Scientific poster (2016) | Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Video | Immunohistochemistry—Tips and tricks for weak or no stain Immunohistochemistry application, offers a spatial visualization and location of an antigen in tissue sections | antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection |
| Video | Immunohistochemistry—Tips and tricks for high background Immunohistochemistry application offers a spatial visualization and location of an antigen in tissue sections. This video offers troubleshooting tips for issues falling into the "high background" category for IHC | antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection |
For Research Use Only. Not for use in diagnostic procedures.