Figure 1. Stages of the adapted cancer-immunity cycle that can be impacted by CAR T-cell therapy.
Comprehensive solutions to support CAR T-cell therapy research from T-cell engineering to sequencing
Adoptive cell transfer is a rapidly emerging area of immuno-oncology research. This approach involves collecting, manipulating, activating, and transferring a subject’s immune cells to treat cancer. A widely studied approach to cell therapy is chimeric antigen receptor T-cell therapy, also known as CAR T-cell therapy.
Many immuno-oncology therapeutics are mass-produced antibody or fusion proteins. CAR T-cell therapy breaks that mold by utilizing a genetically modified patient T cell that attacks and kills the cancer cells. T cells do not normally identify cancer cells as foreign bodies, which would mark them for destruction. For immunotherapy to work, T cells are genetically manipulated to enable them to identify the cancer cells as an enemy and kill them as they would other foreign bodies. The T cells are genetically modified with CARs containing antibody-based recognition domains directed against cell-surface antigens linked to intracellular signaling sequences to overcome the cancer cell’s tolerance. Once created, these modified T cells are reintroduced into the subject along with chemotherapy.
Analyzing the immune repertoire to capture the diversity of T-cell receptor (TCR) rearrangements can help you make significant progress in immuno-oncology research (Figure 1). Explore workflow solutions from engineering to sequencing through cell media and reagents.
Click image to enlarge
Featured products
Our Gibco Cell Therapy Systems (CTS) products facilitate a seamless transition from research to commercialization.
Designed to measure T-cell diversity and clonal expansion by sequencing T-cell receptor beta chain rearrangements
Discover the only complete genome-editing solution designed to expedite your CAR T-cell research with less effort.
Explore our CAR T-cell therapy solutions and technologies
Sequencing
Learn more about our sequencing solutions including NGS and Sanger sequencing.
- Next-generation sequencing (NGS) using the Ion GeneStudio S5 Series sequencers and the Ion Torrent Oncomine TCR Beta-LR Assay
- Sanger sequencing with the Applied Biosystems SeqStudio Genetic Analyzer
Quantitative protein analysis
We offer quality Invitrogen immunoassays designed to help you easily quantify checkpoint protein biomarkers with confidence.
Singleplex quantitation of proteomic biomarkers
- Validated ELISA kits—complete and ready to use, designed to enable accurate protein quantitation of immune-related cancer targets. We also offer simplified workflow and high-sensitivity options.
- Robust, reliable microplate readers—from the original inventors of microplate readers, choose from a lineup of tried and true Thermo Scientific dedicated and multimode readers, all powered by the same intuitive SkanIt Software.
- ProQuantum Immunoassays—when working with very limited samples, this unique platform offers high-sensitivity with a streamlined workflow, available with popular targets used in I/O research.
Multiplex quantitation of proteomic biomarkers
- ProcartaPlex preconfigured panels—profile more biomarkers simultaneously, save time and sample
- Immuno-Oncology Checkpoint 14-Plex Human ProcartaPlex Panel 1—custom mix for I/O research
- Immuno-Oncology Checkpoint 14-Plex Human ProcartaPlex Panel 2—custom mix for I/O research
- ProcartaPlex panel configurator tool—choose your specific I/O targets and have us blend and validate a custom multiplex panel for you with up to 80 analytes
Engineering
Find tools and reagents needed for T-cell engineering and manipulation.
- PureLink Fast Low Endotoxin kits—for plasmid DNA isolation
- LV-MAX Lentiviral Production System—for high-yield production
- TrueGuide Synthetic gRNA and TrueCut Cas9 Protein v2—for maximum editing efficiency
- Neon Transfection System—for efficient delivery of
T cells - GeneArt Gene-to-Protein Services—for CAR synthesis
Cell isolation, activation, and expansion
Leverage magnetic beads and reagents, trusted solutions for ex vivo T cell isolation, activation, and expansion for immunotherapy.
Cell counting
Automated, highly accurate cell counting to minimize variability with the Countess II FL Automated Cell Counter.
Adoptive cell therapy
From isolation and activation to 3D cellular assays, learn more about CAR T cell and other adoptive cell therapy workflows.
Immuno-Oncology Product Resource Guide
The Immuno-Oncology Product Resource Guide is an introduction to immuno-oncology research, covering key approaches such as CAR T-cell therapy research and solutions for each application area.
Resources
Support
For Research Use Only. Not for use in diagnostic procedures.