If you agree that your CHO-derived biologic shouldn’t be subject to development constraints imposed by others, break free with Gibco Freedom Cell Line Development Kits. Gain commercial licensing that’s simple, flexible, and without royalties.
Gibco Freedom kits, developed in collaboration with ProBioGen AG, contain all the components for beginning-to-end cloning and expression, within a short development time (<6 months from gene of interest to clone). Contact us about our 40% discount program on the ExpiCHO-S kit or CHO-DG44 kit today.
Freedom Kit benefits include:
- Regulatory-friendly, cGMP-banked cell lines
- Complete protocol provided
- Fast and efficient workflow for the creation of stable clones
- Proven for IgG production
- Flexible, royalty-free commercial licensing options with no annual fees
- High titers (3–5 g/L in fed-batch cultures)*
*When using Freedom ExpiCHO-S Kit as recommended. Results may vary, based on process conditions.
Licensing simplicity. Workflow scalability. Superior technical support.
Your Freedom kit includes support from the Gibco Field Application Scientist (FAS) team—experts in biopharmaceutical processes and the use and applications of Gibco products and services. The FAS team has broad experience in cell culture, process characterization, scale-up, analytics, product development, process optimization, and tech transfer. Guidance and step-by-step support can be provided to you throughout the cell line development workflow.
| Freedom from royalties? | Yes |
| Freedom from annual maintenance fees? | Yes |
| Freedom to use any contract manufacturer? | Yes |
| Freedom to host cell line for multiple projects?* | Yes |
| Freedom to modify the host cell line? | Yes |
| Freedom to modify the purchased vector? | Yes |
| Freedom to conduct process optimization? | Yes |
| Freedom to request support when desired? | Yes |
| Freedom to partner with others within field of use?* | Yes |
| *Some sale and distribution restrictions may apply |
Details of our three kits
Licensing questions? Contact us for license terms.
Freedom ExpiCHO-S kit | Freedom CHO-DG44 kit | Freedom CHO-S kit | |
Catalog Number | A46847 | A1373701 | A136960 |
Parental Cell Line | cGMP ExpiCHO-S cells | cGMP CHO-DG44 cells | cGMP CHO-S cells |
Medium | ExpiCHO expression medium and ExpiCHO stable production medium | CD DG44 and CD OptiCHO | CD FortiCHO |
Growth rate | <24 hours | >30 hours | <24 hours |
Vector | Freedom pCHOm 3.1and Freedom pCHOp 3.2 | pcDNA 3.3-TOPO and pOptiVEC-TOPO | Freedom pCHO 1.0 |
Primary application | mAb and recombinant protein | mAb and recombinant protein | mAb (IgG)
|
| Amplification (with Methotrexate) | Yes | Yes | No |
Timeline – transfection to stable clone | ~24 to ~36 weeks | ~36 weeks | ~24 weeks |
Complete Manual | Yes | Yes | Yes |
Support | Yes | Yes | Yes |
Commercial licensing | Yes | Yes | Yes |
|
Related resources
- Brochure: Gibco Media by Design Services
- eBook: Freedom ExpiCHO-S Kit FAQs
- Flyer: Freedom cell line development kits
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