General Sandwich ELISA Protocol
Find protocols for a standard sandwich ELISA using a 96-well plate for the detection techniques—colorimetric, chemiluminescent, and fluorescent detection.
ELISA Instrumentation and Equipment |
Enhance the efficiency and ease of your ELISA workflow with our comprehensive range of instruments and equipment. Thermo Fisher Scientific offers a diverse selection of solutions including ELISA microplate readers, ELISA plate washers, single channel and multichannel pipettes, dispensers and pipette tips, and more. Additionally, we provide laboratory equipment such as centrifuges and cold storage, tailored to meet all your ELISA requirements. Discover how our top-quality products can help streamline your ELISA experiments.
Lightweight, ergonomic single channel ClipTip pipettes and Finnpipettes are available in variable and fixed volume models to enable consistent and reproducible results.
Robust and durable multichannel ClipTip pipettes and Finnpipettes are available in 6, 8, 12 or 16 channels. Choose from our wide selection of manual and electronic pipettes to help reduce pipetting time by up to 90%.
A broad range of filtered, non-filtered, and ART self-sealing barrier pipette tips are available. Explore our unique ClipTip interlocking technology for best possible accuracy and precision.
Our fast and reliable Multidrop dispensers can help save time at the bench. Achieve excellent precision and reproducibility by filling plates in seconds, enabling faster test runs and increased productivity. The compact design of our Multidrop dispensers enables easy placement on the bench, while the user-friendly interface helps ensure effortless setup and programming. Multidrop reagent dispensers with peristaltic pump dispensing technology utilize detachable, autoclavable Multidrop cassettes for dispensing reagents, diluents, cells, and beads.
Wash 96-well plates securely with our Wellwash Microplate Washer, an easy-to-use, reliable microplate-strip washer for routine ELISA applications. The Wellwash features a graphical user interface, local language versions, and USB port for optimal ease-of-use and convenience.
Wash 96- or 384-well plates securely with our Wellwash Versa Microplate Washer, an easy-to-use microplate-strip washer for routine ELISA applications. Designed for high performance and versatility, enjoy the ease-of-use and convenience provided through a graphical interface, local language version, and USB port. Automated workflows help save time with robot compatibility offered by Wellwash Versa Microplate Washer.
A robust and reliable filter-based instrument that can be used for a wide variety of research and routine applications, including ELISA, growth curves, protein quantification, endotoxin and cytotoxicity assays. It can be used as a stand-alone instrument or under PC control with intuitive SkanIt Software. Multiskan FC offers proven performance and reliable day-to-day results through optical design and built-in self-diagnostics.
A UV/Vis microplate reader designed for photometric or turbidimetric research applications, particularly DNA, RNA and protein analysis. It enables kinetic, spectral, and endpoint measurements for a variety of applications and separate optimized measurement modes for absorbance and turbidimetric measurements. Multiskan SkyHigh offers fast reading speed and an optional, easy-to-use, graphic touchscreen and multiple connectivity options (USB, computer, or cloud).
Learn more about Multiskan SkyHigh Microplate Spectrophotometer
A versatile, entry-level multimode microplate reader that can be used for a variety of UV/Vis absorbance, fluorescence, and luminescence microplate assays. The Varioskan ALF multimode reader is fast, flexible, easy-to-use and offers multiple detection modes with top reading that enables basic microplate reader applications, including nucleic acid and protein quantification, bacterial growth curves, ELISA, and cell viability. It is a plug-and-play solution that gives you full control of your microplate reader assays.
Designed to suit a wide variety of needs, the Varioskan LUX Multimode Microplate Reader has a flexible range of measurement modes. The instrument simplifies measurement setup with automatic dynamic range selection, and its smart safety controls help you avoid experimental errors. It features five detection modes (absorbance, fluorescence, luminescence, TRF, and AlphaScreen modules) and five measurement modes (end-point, kinetic, spectral, multipoint, and kinetic-spectral). The Varioskan LUX Multimode Microplate Reader raises the bar to enable reliability and ease.
Separations are a critical step in your workflow, thus it is important to consider the centrifuge requirements and technical specifications for your applications, from selecting the appropriate speed and g-force to exploring our latest trends in centrifugation. A wide array of our centrifuges and their innovative rotors are available for your processing needs, supporting labware from microplates and microtubes to large-capacity bottles—all designed to deliver outstanding performance, spin after spin.
Our trusted cold storage and sample preparation solutions offer sample protection for a wide array of sample types and a large range of storage conditions from 4°C high-performance laboratory refrigerators to –196°C cryogenic freezers. Discover why researchers worldwide protect billions of samples with our cold storage equipment and consumables.
Learn about our ELISA kit formats including complete, ready-to-use kits as well as preoptimized reagents to design your own assay.
Find HRP and other enzyme substrates for ELISA applications in a range of sensitivities for different methods including colorimetric, chemiluminescent, and fluorescent detection.
Select from a wide variety of easy-to-use and reliable blocking buffers and reagents for ELISA applications.
Choose from an array of microplates with a variety of surfaces optimized to capture the biomolecule of your choice.
Find protocols for a standard sandwich ELISA using a 96-well plate for the detection techniques—colorimetric, chemiluminescent, and fluorescent detection.
Find the right immunoassay for your research needs.
Immunoassay guide to protein quantitation.
Explore our immunoassays for different research areas, including immuno-oncology, cell signaling, neurobiology, inflammation and cytokine storm, hallmarks of cancer, car t cell therapy and more.
For Research Use Only. Not for use in diagnostic procedures.