True to its name, the High-Capacity cDNA synthesis kits produce high quality first-strand cDNA from total RNA. The High-Capacity cDNA Reverse Transcription Kit (formerly the High-Capacity cDNA Archive Kit) is designed for synthesizing cDNA for short- or long-term archiving. The kits yield quantitative reverse transcription products from RNA inputs ranging between 0.02 and 2 μg in a 20 μL reaction; it can be scaled up to 10 μg of input RNA in a 100 μL reaction. Downstream applications include real-time PCR, standard PCR, and labeling for microarray analysis.
High-capacity cDNA synthesis kits: formats
The High-Capacity cDNA reverse transcription kits are available in multiple formats:
Benefits of the High-Capacity RNA-to-cDNA Kit
The High-Capacity RNA-to-cDNA kit consolidates the reagents for reverse transcription reaction into two tubes: 2X RT buffer mix and 20X enzyme mix, which contains reverse transcriptase and RNase inhibitor. These components have been optimized to provide high cDNA yields for real-time gene expression analysis. The two-tube format streamlines the workflow by minimizing the number of pipetting steps and is designed for optimum performance in real-time PCR with TaqMan Fast Advanced Master Mix and PowerUp SYBR Green Master Mix.
Comparison of cDNA synthesis kits for qPCR
For exceptional performance, explore SuperScript IV Reverse Transcriptase—available as a standalone enzyme, first-strand cDNA synthesis kit, master mix for qRT-PCR applications, and one-step RT-PCR system to suit all your research needs.
Reverse transcriptases: Selection tool
Use the selection tool to find the right product for your cDNA synthesis experiments
| High-Capacity cDNA Reverse Transcription kit | High-Capacity RNA-to-cDNA kit | SuperScript IV VILO Master Mix | SuperScript IV VILO Master Mix with ezDNase Enzyme | |
| Application | Value choice for routine applications | cDNA synthesis components in two-tube format | Exceptional master mix for two-step RT-qPCR | Exceptional master mix for two-step RT-qPCR with fast and easy gDNA removal |
| Reverse transcriptase | MultiScribe | MultiScribe | SuperScript IV | SuperScript IV |
| Optimal reaction temperature | 37°C | 37°C | 50°C | 50°C |
| Reaction time | 120 min | 30 to 60 min | 10 min | 10 min |
| Ability to work with degraded or inhibitor-containing RNA | Low | Low | High | High |
| Reduced RNase H activity | No | No | Yes | Yes |
| Primers included | Random primers | Random primers | Random primers and oligo(dT)20 | Random primers and oligo(dT)20 |
| Integrated gDNA removal | No | No | No | Yes* |
| Format | RT reaction components in separate tubes | RT reaction components in two-tube format | All RT reaction components premixed | All RT reaction components premixed |
*Formats supplied with ezDNase enzyme
Resources
Reverse transcriptase selection tool
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Reverse transcriptase webinars
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Reverse transcriptase literature
Access white papers and technical and application notes for insights on cutting edge reverse transcription technology.
Reverse transcriptase videos
Watch instructional videos on troubleshooting tips, how-to videos, and explainers related to cDNA synthesis.
Reverse transcriptase education
Review educational resources on topics including reverse transcription basics, cDNA synthesis, enzyme selection, troubleshooting tips, and cDNA applications in molecular biology.
Support
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Tm calculator
Handy tool to calculate Tm (melting temperature) of primers and annealing temperatures.
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For Research Use Only. Not for use in diagnostic procedures.