When analyzing leukocytes by flow cytometry, efficient cell lysis of erythrocytes from whole blood samples must performed prior to analysis. We offer Cal-Lyse premixed lysis solution for erythrocyte lysing for flow cytometry and other applications.
Cal-Lyse and High-Yield Lysis Solutions
Cal-Lyse premixed lysis solution is specifically formulated to lyse erythrocytes following monoclonal antibody staining of whole blood or bone marrow. Treatment with this reagent leads to both the lysis of red blood cells and the fixation of white cells. Treatment does not affect fluorophore-labeled antibodies bound to leukocytes, and leaves morphological scatter characteristics of leukocytes intact.
High-Yield Lyse is a premixed, fixative-free erythrocyte-lysis solution that can be used to help eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with fluorophore-conjugated monoclonal antibodies; there is no need for a wash step.
Cal-Lyse reagent selection guide
| Product | Class | Quantity | Cat. No. |
|---|---|---|---|
| Cal-Lyse RBC Lysis Solution | IVD* | 25 mL | GAS010 |
| Cal-Lyse RBC Lysis Solution | IVD* | 100 mL | GAS010S100 |
| High-Yield Lyse | GPR* | 500 mL | HYL250 |
| *IVD = in vitro diagnostics; GPR = general purpose reagent | |||
Resources
Fluorophore and reagent selection guide for flow cytometry
Download Flow Cytometry Protocols Handbook
Spectral Flow Cytometry Fundamentals
Invitrogen eBioscience Resources—Selection guides, Best Protocols, product performance and more.
Intracellular Staining for Flow Cytometry How-To Video—for detecting cytokines and intranuclear markers.
Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.
Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.
5 Steps Resources
Support
Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.
Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.