Multiplexing Single Cell Proteomics: A Marriage of Sensitivity and Throughput
Dr. Erwin Schoof, Head of Proteomics Facility,
Technical University of Denmark Postdoctoral Fellow, Copenhagen University
Go beyond with Orbitrap Tribrid MS for large and small molecule analysis
Built on revolutionary Thermo Scientific Tribrid architecture that combines the best of quadrupole, Orbitrap, and linear ion trap mass analyzers, Orbitrap Tribrid mass spectrometers enable high throughput analyses of challenging samples.
These include accurate and precise quantitation of low-abundance peptides in complex matrices, characterization of structural isomers, from drug metabolites to intact proteins, resolution of isobaric species, and native protein complexes structure characterization.
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Look inside an Orbitrap Tribrid Mass Spectrometer
The architecture inside of an Orbitrap Tribrid mass spectrometer enables a broad range of experimental applications from discovery to profiling and quantification, expediting scientific advancements while offering experimental flexibility, all on a single instrument.
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Explore our Orbitrap Tribrid instruments
Look inside the novel Orbitrap IQ-X Tribrid mass spectrometer.
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Orbitrap Ascend Tribrid MS
Scale up your science. With new capabilities for multiplexed quantitative proteomics and native protein characterization, the Thermo Scientific Orbitrap Ascend Tribrid mass spectrometer delivers ultimate experimental throughput, versatility, and ease-of-use. Quantify more samples at lower concentrations using intelligent acquisition and faster Real-Time Search. Achieve greater coverage using a revolutionary new hardware design featuring two ion routing multipoles. Or characterize the largest biopharmaceuticals with optional mass range up to m/z 16,000. All while producing more with less instrument setup featuring automated Auto-Ready ion source for calibration and pre-built, optimized method templates to maximize convenience and ease-of-use. Be ready for tomorrow’s most challenging demands
“One thing on the Orbitrap Ascend Tribrid MS that is really exciting is the new ion routing multiples, and how by moving the collision cell forward, the speed of the system has gotten even faster from the last model. That means that we can dig deeper in a sample, and we can detect more things in a shorter period of time”
Joshua Coon, PhD Principal Investigator, Coon Laboratories, University of Wisconsin-Madison
One Cell at a Time: Single Cell Proteomics Becomes a Reality
Dr. Ryan Kelly, Associate professor
Chemistry and Biochemistry, Brigham Young University
Multiplexing Single Cell Proteomics: A Marriage of Sensitivity and Throughput
Dr. Erwin Schoof, Head of Proteomics Facility,
Technical University of Denmark Postdoctoral Fellow, Copenhagen University
One Cell at a Time: Single Cell Proteomics Becomes a Reality
Dr. Ryan Kelly, Associate professor
Chemistry and Biochemistry, Brigham Young University
Orbitrap IQ-X Tribrid MS
Thermo Scientific Orbitrap IQ-X Tribrid Mass Spectrometer is our premier Orbitrap Tribrid MS dedicated to the analysis of small molecules. Utilizing multiple modes of fragmentation, intelligent data acquisition with AcquireX workflow, intelligent MSn powered by Real-Time Library Search, and annotation of unknowns with the mzLogic algorithm, it facilitates comprehensive structural characterization of unknowns in a wide variety of applications ranging from forensics to metabolomics.
Researchers across the globe are making use of the Thermo Scientific Orbitrap ID-X Tribrid mass spectrometer to study metabolic perturbations caused by Covid-19. Ivayla Roberts et al… uncovered a combination of metabolites that are prognostic markers of disease severity and whether the patient are likely to survive.
High-throughput Identification of Novel Tomato Flavonoids Using a Structure-based Profiling and Annotation Workflow
Dr. Sheng Zhang, Director
Proteomics Facility and Metabolomics Facility, Cornell University
High-throughput Identification of Novel Tomato Flavonoids Using a Structure-based Profiling and Annotation Workflow
Dr. Sheng Zhang, Director
Proteomics Facility and Metabolomics Facility, Cornell University
Orbitrap Fusion Lumos Tribrid MS
Recently, the Orbitrap Fusion Lumos mass spectrometer was used to characterize DNA-protein crosslinking in Escherichia coli engineered to use methanol as a source of carbon. Researchers from UCLA showed that cultures inoculated from the stationary phase exhibited exceedingly long lag phases. Identification and quantification of DNA-crosslinked proteins provided insight on the possible causes of cell death and potential disruption to the transcription and translation processes that would explain this observation.
Orbitrap Fusion Tribrid MS
Proteomic Music from Biological Noise: Improvement of sensitivity and comprehensiveness of proteomic analyses using a novel FAIMS Pro Interface
Dr. Pierre Thibault, Professor
Department of Chemistry, Faculty of Arts and Sciences, University of Montreal
Proteomic Music from Biological Noise: Improvement of sensitivity and comprehensiveness of proteomic analyses using a novel FAIMS Pro Interface
Dr. Pierre Thibault, Professor
Department of Chemistry, Faculty of Arts and Sciences, University of Montreal
Related products and technologies
High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS selectivity successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
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