Hot-start PCR prevents the amplification of non-specific products, amplifies low abundance targets and offers convenient room temperature reaction setup. We offer different hot-start DNA polymerases to support your everyday research needs. This includes Thermo Scientific DreamTaq Hot Start DNA Polymerase, which is an enhanced hot start Taq DNA polymerase suitable for most PCR applications. DreamTaq Hot Start offers higher sensitivity, specificity, and yields compared to conventional hot start Taq DNA polymerase.
Choose a hot-start DNA polymerase
| For everyday research | For fast PCR | |
|---|---|---|
| DreamTaq Hot Start DNA Polymerase | Phire Hot Start II DNA Polymerase | |
| Enhanced specificity | Yes | Yes |
| Sensitivity | 3 pg | 100 pg |
| Amplicon size–genomic/phage DNA | ≤6 kb/20 kb | ≤7.5 kb/20 kb |
| Activation time | Immediate | Immediate |
| Cycling time | 60 s/kb | 10–15 s/kb |
| 3′A overhang or blunt | 3′A | Blunt |
| Stand-alone enzyme | ||
| Master mix |
*Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.
Additional hot-start DNA polymerases
Resources
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