Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
- I am interested in expressing some functional membrane proteins in mammalian cells. Do you offer a system that will allow me to do this?
- Is there a positive control included with the MembranePro™ Functional Protein Expression Kit or the Expi293™ MembranePro™ Expression System?
- Can I use a different expression vector with the MembranePro™ Functional Protein Expression Kit or the Expi293™ MembranePro™ Expression System?
- Can I use an existing stable cell line expressing a GPCR of interest with the MembranePro™ Functional Protein Support Kit?
- Does the expression vector in the MembranePro™ Functional Protein Expression Kit offer a His tag?
Troubleshooting
- Your MembranePro™ Functional Protein Expression Kit/Expi293™ MembranePro™ Expression System does not include a positive control. How do I tell if MembranePro™ particles were formed?
- Can I use a different expression vector with the MembranePro™ Functional Protein Expression Kit or the Expi293™ MembranePro™ Expression System?
- I had a problem growing up the 293T cells provided in the MembranePro™ Functional Protein Expression Kit. Can I use a different cell line?
- The Expi293F™ cells supplied with the Expi293™ MembranePro™ Expression System had poor viability upon thawing. Can I use a different cell line?
Guides and Tools
Search our database for Product Documents: Manuals, Vector Maps, Citations and References
Search our database for Quality and Safety Documents: MSDS, COA
- Overview: MembranePro™ Expression Systems
- Overview: Expi293™ MembranePro™ Expression System
- Learning Center: Protein Biology
- Protein Biology Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Guide: Protein Expression Handbook
- Video: Cell Culture Basics from Gibco®
- Vector Selection Tool
- Vector NTI: a bioinformatics software package
- Gibco® Cell Culture Companion: An app designed to capture and store vital experiment information as you count cells, perform cell culture calculations, and generate growth curves.
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more. Also includes a Transfection Calculator you can use to simplify transfection experimental setup.
Gibco® MediaExpress™ (non-cGMP) Custom Media
Specifically designed for small-scale, non-cGMP custom media orders when speed matters most; offers Gibco® quality media in small batches for quick turnaround and smooth transition to cGMP scale-up.
GeneArt® Cell Lines and Proteins Services
GeneArt® Cell Lines and Proteins are a suite of service offerings designed to help facilitate protein expression in mammalian cell lines. We can help with every step of the process, including gene design, synthesis, cloning, protein production, and even stable cell generation. Alternatively, we can help with just the parts of the process you request.
Protein Expression and Production Services
Have us utilize our extensive suite of expression vectors and host systems to deliver the necessary protein yield for your application. We offer protein expression and production services in baculovirus and mammalian host systems, as well as protein purification and analysis services.
For Research Use Only. Not for use in diagnostic procedures.