“…it became evident that this can be a very useful tool, not only for training, but in production.”
Nik Matthews, Tumor Profiling Unit, Institute for Cancer Research, UK
The Invitrogen Collibri portfolio of library prep kits and accessories generate libraries more quickly, with less bias, in order to match the speed and capacity of Illumina instruments. Advantages available only from Invitrogen Collibri Library Prep Kits:
- Visual feedback for higher library prep success
- Shorter total workflow times, greater support
- Reduced bias in genomic interpretation from whole genome sequencing
- Clean data reveals transcriptome complexity from whole transcriptome sequencing
Research SARS-CoV-2 mutations and gene expression profiles
Collibri Library Prep kits for Illumina Systems enhance NGS-based viral surveillance with higher quality sequences and rapid protocols.
- Identify emerging strains
- Rapidly generate gene expression profiles
- Research target sequences for vaccine or therapeutic development
Visual feedback at each step in the protocol indicates proper addition and mixing of reagents
Video: Improve success rates of library generation using visual feedback
Figure 1. An example of the Collibri PS DNA Library Prep Kit for Illumina systems workflow in which each reagent contains a unique tracking dye that provides visual feedback of proper reagent addition and mixing.
Researchers value the visual feedback in Collibri library prep kits
Shorter total workflow times, greater support
The Collibri library prep workflows match the pace needed to efficiently utilize Illumina systems. Reduce time spent on placing orders, managing inventory and contacting technical support from multiple companies. Thermo Fisher Scientific offers technical and ordering support for most steps in the total pre-sequencing workflow for improved efficiency of your lab operations.
Whole-genome sequencing PCR-free library prep workflow
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Figure 2. The Collibri PCR-Free PS DNA Library Prep Kit protocol is completed in 1.5 hours, reducing total workflow time to ~6.5 hours.
RNA-seq library prep workflow
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Figure 3. The Collibri Stranded RNA Library Prep Kit with rRNA Depletion protocol is completed in 6.5 hours, reducing total workflow time to ~10.5 hours.
Visual feedback is provided by the entire Collibri portfolio of library prep kits and accessories
Whole-genome sequencing
Reduce bias in genomic interpretation
RNA sequencing
Clean data reveals transcriptome complexity
Library quantification
Determine the concentration of your Illumina NGS libraries
Library amplification
Amplify libraries for sequencing on Illumina systems
Technical Note: Understanding library quantification assays for next-generation sequencing applications
NGS libraries must be carefully quantified and normalized so that each sample achieves the required number of reads.
Resources
App note
Brochures
DNA sequencing
Technical notes
All sequencing methods
Technical notes
RNA sequencing
Technical notes
For Research Use Only. Not for use in diagnostic procedures.