Figure 3. Expi293 Expression System protocol.
Precise protein expression is all in the fold
For structural biologists, insoluble and misfolded proteins mean starting over. The Expi293 Expression System is precisely designed with suspension 293 cells, optimized medium, and transfection reagent to enable native folding and maximum protein yields. Now your structural study can get the biological relevance it deserves.
Expi293 Transient Protein Expression System for structural biology and inducible expression
Experience the Gibco Expi293 suite of structural biology products, precisely designed to enable uniform glycosylation, tight control of expression, and protein labeling in the first-ever HEK293 suspension system. Achieve rapid, high-yield protein production with three specialized Expi293F cell lines (GnTI-, Inducible, and Inducible GnTI-) and a Met (-) protein labeling kit.
Transient HEK 293 Expression Protocol
Learn how to produce recombinant proteins with the Gibco Expi293 Expression System and achieve gram per liter protein yields in a transient HEK 293 system.
Set up your lab for mammalian transient protein expression
The Expi293 Expression System is precisely designed with suspension 293 cells, optimized medium, and transfection reagent to enable native folding and maximum protein yields.
Rapid and ultrahigh-yield protein production in mammalian cells
The Expi293 Expression System is a rapid method of producing milligram to gram quantities of recombinant protein in mammalian cells (Figure 1).
Figure 1. Expression of human IgG and Fc-tagged Cripto achieve expression levels of over 1 g/L in the Expi293 Expression System.
Study correctly folded, active protein and assembled complexes
The Expi293 Expression System is based on expression in mammalian 293 cells, which offers the benefits of native folding and posttranslational modifications. The two figures below show purification of the HDAC3/SMRT-DAD complex from 293 cells and E. coli. The 293 cells yield a stable pure HDAC3 complex (Figure 2A), whereas expression in E. coli (Figure 2B) does not.
Figure 2. Purification of the HDAC3/SMRT-DAD complex from (A) mammalian 293 cells and (B) E. coli. In both cases the protein complex was purified using an affinity tag on SMRT: Flag-tag for the mammalian-expressed proteins, and GST-tag for the E. coli-expressed proteins. The HDAC3 was untagged.
Fast and simple protocol—no more difficult than E. coli expression
Eliminate the hassle of working with baculovirus insect expression systems or creating stable mammalian cell lines. The Expi293 Expression System is quick and easy and is designed to provide ultrahigh protein yields in just a week (Figure 3).
Expi293 protein expression data
The table below highlights yields of various proteins produced by the Expi293 Expression System.
| Description | Gene symbol | Accession number | Expi293 system yield (mg/L) |
|---|---|---|---|
| Pleckstrin, mRNA (cDNA clone MGC:17111 IMAGE:4341823), complete CDS | PLEK | AAH1549.1 | 5,610 |
| Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 1, mRNA | STAT3 | NP_644805.1 | 2,139 |
| BH3 interacting domain death agonist (BID), transcript variant 2, mRNA | BID | NP_001187.1 | 972 |
| RAB38, member RAS oncogene family (RAB38), mRNA | RAB38 | NP_071732.1 | 702 |
| S100 calcium binding protein A4 (S100A4), transcript variant 1, mRNA | S100A4 | NP_002952.1 | 609 |
| NAD(P)H dehydrogenase, quinone 1 (NQ01) transcript variant 1, mRNA | NQ01 | NP_000894.1 | 525 |
| Prosaposin (PSAP), transcript variant 1, mRNA | PSAP | NP_002769.1 | 524 |
For Research Use Only. Not for use in diagnostic procedures.