Expanding options for violet laser-excitable antibody conjugates
Super Bright dyes vs. Brilliant Violet dyes for flow cytometry
- Super Bright antibody conjugates have comparable brightness to Brilliant Violet™ conjugates
- Both can be utilized in flow cytometry applications, typically without a need to adjust standard workflows
- Both dye families are violet laser–excitable polymer dyes
- Both require use of a special buffer to reduce nonspecific dye-dye interactions when two or more polymer-dye antibody conjugates are used
Super Bright dye antibody conjugates selection guide
| Dye | Super Bright 436 | Super Bright 600 | Super Bright 645 | Super Bright 702 | Super Bright 780 |
|---|---|---|---|---|---|
| Comparable to | Brilliant Violet™ 421 | Brilliant Violet™ 605 | Brilliant Violet™ 650 | Brilliant Violet™ 711 | Brilliant Violet™ 786 |
| Brightness |
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| Excitation | violet (405 nm) | violet (405 nm) | violet (405 nm) | violet (405 nm) | violet (405 nm) |
| Emission | 436 nm | 600 nm | 645 nm | 702 nm | 780 nm |
| Suggested bandpass filter | 450/50 | 610/20 | 660/20 | 710/50 | 780/60 |
| More about the dye | View the Super Bright 436 dye dashboard | View the Super Bright 600 dye dashboard | View the Super Bright 645 dye dashboard | View the Super Bright 702 dye dashboard | View the Super Bright 780 dye dashboard |
| Comparative data | See data | See data | See data | See data | See data |
Resources
Support
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.