Dynabeads for human T Cell expansion
Ready-to-use Dynabeads will allow you to optimize ex vivo T cell activation and proliferation in translational research, while preserving T cell viability and optimal immune function. Simultaneous signaling to TCR/CD3 and CD28 triggers a physiological activation and expansion of human T cells. You can also stimulate and expand human antigen-specific T cell lines or clones using Dynabeads Human T-Activator CD3/CD28/CD137.
This simple, efficient and consistent technology out-performs and replaces traditional home-brew methods for generic activation based on antigen-presenting cells (APCs), mitogens (e.g. ConA, PHA), soluble/plate-bound antibodies, or chemical activators, and is well documented in published literature. Expansion and re-infusion of genetically engineered T cells has the potential to revolutionize the treatment of leukemia and related blood cancers. A clinical research grade version is also available, allowing you to move from mouse studies to clinical research using the same technology platform.
Selection guide for human T cell activation and expansion
| Activation and expansion of polyclonal T cells | Activation and expansion of regulatory T cells | Expansion of antigen-specific T cells | Isolation and expansion of polyclonal T cells | cGMP-grade beads for isolation and expansion of polyclonal T cells | |
|---|---|---|---|---|---|
| Dynabead Human T-Activator CD3/CD28 | Dynabeads Human Treg Expander | Dynabeads Human T-Activator CD3/CD28/CD137 | Dynabeads Human T-Expander CD3/CD28 | Dynabeads CD3/CD28 CTS | |
| Research grade | Research grade | Research grade | Research grade | Research-grade version of the cGMP-grade product | For research use or non-commercial manufacturing of cell-based products for clinical research |
| Starting samples | Pure T cells isolated from any sample | Pure regulatory T cells isolated from any sample | T cell lines or T cell clones | Pure T cells isolated from any sample, preferably from cryopreserved human PBMC | Cryopreserved human PBMC obtained from leukapheresis product |
| No. of cells processed | 4 x 107 cells / mL beads | 5 x 106 cells / mL beads | 5 x 106 cells / mL beads | 3.3 x 107 cells / mL beads | 1.6 x 108 cells / mL beads |
| Bead:T cell ratio for activation/expansion | 1:1 | 4:1 | 1:10 | 3:1 | 3:1 |
| Applications | Short-term activation or expansion of polyclonal T cells without the need for feeder cells, e.g., for use in transfection/ transduction, T cell receptor signaling, proteomics | Short-term activation or expansion of pre-isolated regulatory T cells without the need for feeder cells, e.g., for use in flow cytometry, MLR, proteomics, gene expression | Expansion of antigen-specific T cells without the use of feeder cells, e.g., for use in flow cytometry, phenotyping, adoptive transfer | Isolation and expansion of CD3+ T cells, or expansion of pre-isolated T cells or T cell subsets without the need for feeder cells | Isolation and expansion of CD3+ T cells or expansion of pre-isolated T cells or T cell subsets without the need for feeder cells |
| Shelf life from production date | 2 years | 2 years | 2 years | 2 years | 2 years |
| Required materials | Magnets: magnets+ rIL-2 (for expansion) | Magnets: magnets+ rIL-2 (for expansion) | Magnets: magnets + rIL-2 | Magnets: DynaMag CTS Magnet (Cat. No. 12102), or visit magnets + rIL-2 | Magnets: DynaMag CTS Magnet (Cat. No. 12102), or visit magnets+ rIL-2 |
| Order | Order | Order | Order | Order |
References
The references listed below are among the many published papers documenting the performance of the Dynabeads technology for T cell activation and expansion:
- Eyquem, J et al. (2017) Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection. Nature 543: 113-117
- Mock, U et al. (2016) Automated manufacturing of chimeric antigen receptor T cells for adoptive immunotherapy using CliniMACS prodigy. Cytotherapy 18: 1002-1011
- Suarez, ER et al. (2016) Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model. Oncotarget 7: 34341-34355
- Jacomet, F et al. (2015) Evidence for eomesodermin‐expressing innate‐like CD8+ KIR/NKG2A+ T cells in human adults and cord blood samples. Eur J Immunol. 45: 1926-33
- Kane, M et al. (2013) MX2 is an interferon-induced inhibitor of HIV-1 infection. Nature 502: 563-566
- Levring, TB et al. (2012) Activated human CD4+ T cells express transporters for both cysteine and cystine. Sci Rep. 2: 266
- Porter, DL et al. (2011) Chimeric Antigen Receptor–Modified T Cells in Chronic Lymphoid Leukemia. N Engl J Med 365:725-733.
- Kalos M et al (2011) T Cells with Chimeric Antigen Receptors Have Potent Antitumor Effects and Can Establish Memory in Patients with Advanced Leukemia. Sci Transl Med 3:95ra73
- Bonyhadi M et al (2005) In vitro engagement of CD3 and CD28 corrects T cell defects in chronic lymphocytic leukemia. J Immunol 174:2366–2375.
- Rapoport AP et al (2005) Restoration of immunity in lymphopenic individuals with cancer by vaccination and adoptive T-cell transfer. Nat Med 11:1162–1163.
- Godfrey WR et al (2004) In vitro expanded human CD4+CD25+ T regulatory cells can markedly inhibit allogeneic dendritic cell stimulated MLR cultures. Blood 104:453–461.
- Coito S et al. (2004) Retrovirus-mediated gene transfer in human primary T lymphocytes induces an activation-and transduction/selection-dependent TCRBV repertoire skewing of gene-modified cells. Stem Cells Dev 13:71–81.
- Berger C et al (2003) CD28 costimulation and immunoaffinity-based selection efficiently generate primary gene-modified T cells for adoptive immunotherapy. Blood 101:476–484.
- Levine BL et al. (2002) Adoptive transfer of costimulated CD4+ T cells induces expansion of peripheral T cells and decreases CCR5 expression in HIV infection. Nat Med 8:47–53.
For Research Use Only. Not for use in diagnostic procedures.