Choosing the correct first-strand gDNA synthesis kit is critical to obtaining high yields of cDNA from a wide range of input RNAs and improved RT-qPCR assays. From a value choice first-strand cDNA synthesis kit to the best performance in master mix with integrated gDNA removal, you have a choice to select the Invitrogen™ or Applied Biosystems™ cDNA synthesis kit to meet your research needs.
Which cDNA synthesis kits for RT-qPCR is right for you?
| Order now | Order now | Order now | |
| Goal | Highest performance & convenience for RT-qPCR | Highest performance with effective removal of gDNA | Value choice for commonly used RNA templates |
|---|---|---|---|
| Product | SuperScript IV VILO Master Mix | SuperScript IV VILO Master Mix with ezDNase | High-Capacity cDNA Reverse Transcription Kit |
| Reverse transcriptase | Invitrogen™ SuperScript™ IV | SuperScript IV | Invitrogen™ MultiScribe™ MuLV |
| Optimal reaction temp | 50°C | 50°C | 37°C |
| Reaction Time | 10 min | 12 min (10 min for RT + 2 min gDNA removal) | 120 min |
| Reduced RNase H activity | Yes | Yes | No |
| Primers included | Random primers & Oligo(dT)20 | Random primers & Oligo(dT)20 | Random primers |
| Integrated gDNA removal | No | Yes | No |
| Protocol | Download protocol | Download protocol | Download protocol |
Other First-Strand cDNA Synthesis Kits for RT-qPCR
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Video: Overcoming RT-qPCR challenges
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