Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
- Why would I pick a yeast expression system for expression of my protein as opposed to expression systems in other hosts?
- What choices do you offer for protein expression in a yeast host system and what are their features?
- What is the main advantage of protein expression in Pichiapastoris compared to that in S.cerevisiae?
- How do I store yeast long-term? Can I freeze them at –80°C like bacteria? What is the shelf life of frozen stocks?
- What selection mechanism does the PichiaPink™ Yeast Expression System use?
Troubleshooting
- My spheroplasting of Pichia worked twice, but hasn't worked since. The OD of the culture simply does not drop.
- I am trying to prepare and transform competent Pichiapastoris cells using the Pichia EasyComp™ Transformation Kit and am getting a low efficiency of transformation. What should I do?
- I am using the pPink-HC vector and the transformation efficiency of the host strain seems to be very low. Why is this?
- I am trying to generate multimers in Pichia and am encountering issues. Can you please help?
- I am trying to prepare and transform competent S. cerevisiae cells using the S. cerevisiae EasyComp™ Transformation Kit and am getting a low efficiency of transformation. What should I do?
Guides and Tools
Search our database for Product Documents: Manuals, Vector Maps, Citations and References
Search our database for Quality and Safety Documents: MSDS, COA
- Overview: Yeast Expression
- Overview: PichiaPink™ Yeast Expression System
- Overview: Traditional Pichia Expression Systems
- Overview: Yeast Culture
- Learning Center: Protein Biology
- Protein Biology Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Guide: Protein Expression Handbook
- Gibco® Cell Culture Companion: An app designed to capture and store vital experiment information as you count cells, perform cell culture calculations, and generate growth curves.
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more.
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