Rapid resequencing of mitochondrial genomes
Mitochondrial DNA sequencing is a useful tool for researchers studying human diseases such as diabetes, certain cancers, and mechanisms of aging. Mitochondrial DNA sequencing is also used in population genetics and biodiversity assessments. Targeted mitochondrial DNA sequencing can be used to detect mutations present in some copies of the mitochondrial genome (heteroplasmic mutations). Finally, mitochondrial DNA sequencing is important for human identification and forensics applications.
Rapid resequencing of mitochondrial genomes for disease research and biodiversity assessment is accomplished using Ion Torrent™ next-generation sequencing.
Mitochodrial sequencing using Ion Torrent™ next-generation sequencing enables:
- Capacity of 16 samples per run with barcoding
- Accurate variant calling, especially in hypervariable regions of mitochondria
Highly targeted mitochondrial DNA sequencing is performed by gold-standard Sanger sequencing, using Applied Biosystems® genetic analyzers. The simple workflow can be performed in less than 5 hours using the Applied Biosystems® 3500 Genetic Analyzer.
Step-by-Step Guide to Mitochondrial Sanger Sequencing Products
 DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: |
 BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. Recommended Products: |
 BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. PCR Clean up and sequencing reaction are perform in a single step. Recommended Products: |
 After the sequencing reaction, it is important to remove unincorporated dye terminators and salts that may compete for capillary electrophoretic injection. Unincorporated terminators can co-migrate with the sequencing template, resulting in basecalling er Recommended Products:Which Sample Cleanup Product Is Right for You?
|
 During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
 Analyze the mitochondrial sequence. Which Data Analysis Software Is Right for You? |
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: |
BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. Recommended Products: |
BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. PCR Clean up and sequencing reaction are perform in a single step. Recommended Products: |
After the sequencing reaction, it is important to remove unincorporated dye terminators and salts that may compete for capillary electrophoretic injection. Unincorporated terminators can co-migrate with the sequencing template, resulting in basecalling er Recommended Products:Which Sample Cleanup Product Is Right for You?
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the mitochondrial sequence. Which Data Analysis Software Is Right for You? |
Step-by-Step Guide to Mitochondrial Sanger Sequencing Products
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: |
BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. Recommended Products: |
BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. PCR Clean up and sequencing reaction are perform in a single step. Recommended Products: |
After the sequencing reaction, it is important to remove unincorporated dye terminators and salts that may compete for capillary electrophoretic injection. Unincorporated terminators can co-migrate with the sequencing template, resulting in basecalling er Recommended Products:Which Sample Cleanup Product Is Right for You?
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the mitochondrial sequence. Which Data Analysis Software Is Right for You? |
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: |
BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. Recommended Products: |
BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. PCR Clean up and sequencing reaction are perform in a single step. Recommended Products: |
After the sequencing reaction, it is important to remove unincorporated dye terminators and salts that may compete for capillary electrophoretic injection. Unincorporated terminators can co-migrate with the sequencing template, resulting in basecalling er Recommended Products:Which Sample Cleanup Product Is Right for You?
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the mitochondrial sequence. Which Data Analysis Software Is Right for You? |
For Research Use Only. Not for use in diagnostic procedures.