The QuantStudio™ 12K Flex Real-Time PCR System helps streamline targeted discovery and validation, pathogen detection, and biomarker identification and screening, at a scale previously unattainable. Achieve the highest sample throughput for mid-density gene expression analysis - in a single day, one user can generate >42,000 gene expression data points without the use of robotics.
- More replicates—each plate generates 2,600 data points, enabling you to include the technical replicates needed for better statistical power
- Simple workflows—each QuantStudio™ 12K Flex OpenArray® Plate, encased in an alloy bottom for easy handling, comes preloaded with the assays of your choice- just add sample and master mix, cycle, and image
- Experimental flexibility—specify the inventoried TaqMan® Gene Expression Assays you need, and select the OpenArray® Plate format that best meets your project needs
- Reliable and economical—TaqMan® MGB probe chemistry in 33 nL reactions reduces reagent usage
TaqMan® OpenArray® gene expression workflow on QuantStudio™ 12K Flex System
Isolate and purify total RNA.
Recommended Products
 | RNA extraction for Real-Time PCR |
Select inventoried TaqMan® Assays and configure the OpenArray® plate layout using the downloadable workbook tool. QuantStudio™ TaqMan® OpenArray® Real-Time PCR Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with master mix in OpenArray® 384-well sample plate.
Recommended Products
 | TaqMan® OpenArray® Real-Time PCR Plates Configure a Gene Expression OpenArray® Plate using pre-designed TaqMan® Gene Expression Assays. OpenArray® Master Mix, Loader Tips & other plastics: |
Reverse transcription: Choosing the correct reverse transcriptase (RT) for cDNA synthesis is critical to obtaining high yields of cDNA to improve the range of your real-time RT-PCR assays. The SuperScript® VILO™ cDNA Synthesis Kit is designed to increase cDNA yields and improve the range of your qRT-PCR assays. You obtain the same relative representation in your cDNA, irrespective regardless of the abundance of your gene of interest.
Recommended Products
 | SuperScript® VILO™ cDNA Synthesis Kit – 50 reactions |
Pre-amplification: Pre-amplification is an optional and intermediate amplification step that makes it possible to perform gene expression analysis starting with a limited quantity of RNA. Pre-amplification enables you to stretch your precious sample into many more real-time PCR reactions.
Recommended Products
Prepare your sample mix: Mix cDNA or the product of your pre-amplification step with TaqMan® OpenArray® Genotyping Master Mix in a TaqMan® OpenArray® 384-well sample plate.
Recommended Products
Load your sample mixes onto the TaqMan® OpenArray® Real-Time PCR Plate using the OpenArray® AccuFill™ System. Next, insert the TaqMan® OpenArray® Real-Time PCR Plate into the OpenArray® case filled with immersion fluid, and seal with adhesive. Run the reactions and collect your data using the OpenArray® Real-Time PCR Instrument.
Recommended Products
| TaqMan® OpenArray® Real-Time PCR Master Mix |
Load sample mixes onto TaqMan® OpenArray® Real-Time PCR Plate using the QuantStudio 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. Perform a real-time plate read using the QuantStudio™ 12K Flex system.
Recommended Products
| QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block |
Acquire, analyze, and manage data quickly and easily. Amplification curves are presented for review, and data is easily manipulated to calculate Ct and Tm, as well as ΔCt and ΔΔCt measurements.
Recommended Products
| ExpressionSuite Software |
Isolate and purify total RNA.
Recommended Products
| RNA extraction for Real-Time PCR |
Select inventoried TaqMan® Assays and configure the OpenArray® plate layout using the downloadable workbook tool. QuantStudio™ TaqMan® OpenArray® Real-Time PCR Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with master mix in OpenArray® 384-well sample plate.
Recommended Products
| TaqMan® OpenArray® Real-Time PCR Plates Configure a Gene Expression OpenArray® Plate using pre-designed TaqMan® Gene Expression Assays. OpenArray® Master Mix, Loader Tips & other plastics: |
Reverse transcription: Choosing the correct reverse transcriptase (RT) for cDNA synthesis is critical to obtaining high yields of cDNA to improve the range of your real-time RT-PCR assays. The SuperScript® VILO™ cDNA Synthesis Kit is designed to increase cDNA yields and improve the range of your qRT-PCR assays. You obtain the same relative representation in your cDNA, irrespective regardless of the abundance of your gene of interest.
Recommended Products
| SuperScript® VILO™ cDNA Synthesis Kit – 50 reactions |
Pre-amplification: Pre-amplification is an optional and intermediate amplification step that makes it possible to perform gene expression analysis starting with a limited quantity of RNA. Pre-amplification enables you to stretch your precious sample into many more real-time PCR reactions.
Recommended Products
Prepare your sample mix: Mix cDNA or the product of your pre-amplification step with TaqMan® OpenArray® Genotyping Master Mix in a TaqMan® OpenArray® 384-well sample plate.
Recommended Products
Load your sample mixes onto the TaqMan® OpenArray® Real-Time PCR Plate using the OpenArray® AccuFill™ System. Next, insert the TaqMan® OpenArray® Real-Time PCR Plate into the OpenArray® case filled with immersion fluid, and seal with adhesive. Run the reactions and collect your data using the OpenArray® Real-Time PCR Instrument.
Recommended Products
| TaqMan® OpenArray® Real-Time PCR Master Mix |
Load sample mixes onto TaqMan® OpenArray® Real-Time PCR Plate using the QuantStudio 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. Perform a real-time plate read using the QuantStudio™ 12K Flex system.
Recommended Products
| QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block |
Acquire, analyze, and manage data quickly and easily. Amplification curves are presented for review, and data is easily manipulated to calculate Ct and Tm, as well as ΔCt and ΔΔCt measurements.
Recommended Products
| ExpressionSuite Software |
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For Research Use Only. Not for human or animal therapeutic or diagnostic use.