Real-Time PCR Reagents and Kits Resources | Thermo Fisher Scientific

Digital and downloadable resources to help with your qPCR research

As veterans and innovators in real-time PCR (qPCR), we have a wealth of information, both technical and educational, to empower your qPCR research. We’ve featured some of the most frequented resources for both master mixes and general qPCR below.

Featured resources

TaqMan & SYBR assays

Learn about the differences between TaqMan and SYBR real-time PCR assays and find out which chemistry is right for your qPCR application.

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Choosing the right dyes for your multiplexing experiment

Tips from the field - our own Field Application Scientist answers frequently asked questions regarding dye selection.

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qPCR handbook

Whether you’re new to real-time PCR or just looking to brush up on your techniques, this go-to educational resource provides guidance on running a qPCR experiment, how-to videos, diagrams, frequently asked questions, and best practices.

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TaqTalk video series

As experts in qPCR, we address your most frequently asked questions and challenges in our video series, Taq Talk, where you can expect to hear anything from qPCR basics and best practices to experiment setup and choosing the right master mix.

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TaqMan master mixes product literature

TaqMan Fast Advanced Master Mix	Product bulletin: TaqMan Fast Advanced Master Mix
	User guide: TaqMan Fast Advanced Master Mix
	Datasheet: TaqMan Advanced Master Mix on the Bio-Rad CFX96 Real-Time PCR System
	Datasheet: TaqMan Advanced Master Mix on the Roche LightCycler 480 Real-Time PCR System

TaqMan Fast Virus 1-Step Master Mix	Product bulletin: TaqMan Fast Virus 1-Step Master Mix
	User guide: TaqMan Fast Virus 1-Step Master Mix
	Product insert: TaqMan Fast Virus 1-Step Master Mix

TaqMan RNA-to-C_t 1-Step Kit	Product bulletin: TaqMan RNA-to-Ct 1-Step Kit
	Application note: Understanding Ct
	Quick reference card: TaqMan RNA-to-Ct 1-Step Kit

TaqMan Sample-to-SNP Kit	Product bulletin: TaqMan Sample-to-SNP Kit
	Quick reference card: TaqMan Sample-to-SNP Kit

SYBR Green master mixes product literature

SYBR Green Master Mixes	White paper: Design and Optimization of SYBR Green Assays

PowerTrack SYBR Green Master Mix	Product bulletin: PowerTrack SYBR Green Master Mix
	Protocol: Guidelines for comparing PowerTrack SYBR Green Master Mix to other SYBR master mixes

PowerUp SYBR Green Master Mix	Product bulletin: PowerUp SYBR Green Master Mix
	Protocol: Guidelines for comparing PowerUp SYBR Green Master Mix to other SYBR master mixes

Power SYBR Green RNA-to-Ct 1-Step Kit	Product bulletin: Power SYBR Green RNA-to-Ct 1-Step Kit
	Application note: Understanding Ct
	Quick reference card: Power SYBR Green RNA-to-Ct 1-Step Kit

High Resolution Melting (HRM)	Product bulletin: MeltDoctor High Resolution Melt Reagents
	Guide: A Guide to High Resolution Melting (HRM) Analysis
	Quick reference card: HRM Genotyping Experiment
	Quick reference card: HRM Methylation Study
	Quick reference card: HRM Mutation Scanning Experiment
	Application note: High Resolution Melt Analysis for Mutation Scanning Prior to Sequencing
	Application note: Methylation Analysis Using Methylation- Sensitive HRM and DNA Sequencing
	Application note: High Resolution Melting for Genotyping Applications
	Application note: High Resolution melt for genotyping in Jatropha plants
	Customer story: How Dr. Birgit Ebner identifies variants by HRM in difficult samples