Because some lots of FBS contain residual amounts of the antibiotic tetracycline (tet), unintended expression can occur in very sensitive tet-inducible gene expression systems. Tetracycline-system approved FBS, also known as tet-free FBS (tetracycline-free FBS), is designed and tested to ensure the absence of detectable tetracycline and tetracycline analogs, making this specialty serum ideal for cell culture applications relying on these expression systems.
Features of Gibco Tet-Approved FBS
- Undergoes functional, cell-based assays to verify absence of tetracycline or its derivatives
- Delivers quicker workflows, reduced background noise, and increased consistency of expression when working with tet-inducible expression systems
- To manage experimental budgets, cells can be maintained in standard FBS and introduced to tet-approved FBS in the critical days prior to running assays
- Also available in the aliquot-free Gibco One Shot FBS 50 mL bottle
Research areas using tet-system approved (tetracycline-free) FBS
Tet-approved FBS can be used in many applications.
| Research area | Example |
|---|---|
| Neuroscience | Produce GABAA receptors for electrophysiological study [1] |
| Cancer research | Quantitatively control PVT1 expression by tet-on in bladder cancer [2] Tetracycline-regulated shRNA expression to apply to monocyte interaction [3] |
Drug screening | Establish cell line with inducible Glutamate receptor for drug screening [4] |
| Vaccine development | Research for the safer vaccinia virus vaccines with tet-inducible system [5] |
| Gene editing | CRISPR/Cas9-mediated control of NFAT5 [6] and PVT1 and ANRIL [7] |
Researcher quotes
Tet-approved FBS: case study results
A biomanufacturing organization looking to improve the safety of cell culture virus production has engineered a cell line that will only permit virus expression in the presence of tetracycline. Replacing traditional FBS with tet-approved FBS (tetracycline-free FBS) improves control over when the virus is produced.
| FBS used | Condition | Result |
|---|---|---|
| Tet-System Approved FBS | -Tet | |
| Tet-System Approved FBS | +Tet | |
| Standard FBS with residual tetracycline | -Tet | |
| Standard FBS with residual tetracycline | +Tet |
Use of tet-approved FBS helps provide optimal control of the virus production.
Tet-system approved FBS—a secure and efficient solution
- Dostalova Z, Liu A, Zhou X, et al. (2010) High-level expression and purification of Cys-loop ligand-gated ion channels in a tetracycline-inducible stable mammalian cell line: GABAA and serotonin receptors. Protein Science 19(9):1728–1738.
- Zhuang C, Li J, Liu Y, et al. (2015) Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells. Oncotarget 6(38):41194–41203.
- Kubala MH, DeClerck YA. (2017) Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment. Journal of Visualized Experiments 129:56333.
- Chruścicka B, Burnat G, Brański P, et al. (2015) Tetracycline-based system for controlled inducible expression of group III metabotropic glutamate receptors. Journal of Biomolecular Screening 20(3):350–358.
- Hagen CJ, Titong A, Sarnoski EA, et al. (2014) Antibiotic-dependent expression of early transcription factor subunits leads to stringent control of vaccinia virus replication. Virus Research 181:43–52.
- Kang K, Huang L, Li Q, et al. (2019) An improved Tet-on system in microRNA overexpression and CRISPR/Cas9-mediated gene editing. Journal of Animal Science and Biotechnology 10(43).
- Peng L, Pan P, Chen J, et al. (2018) A tetracycline‑inducible CRISPR/Cas9 system, targeting two long non‑coding RNAs, suppresses the malignant behavior of bladder cancer cells. Oncology Letters 16:4309–4316.
Resources
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