This section features educational resources and tools to help you choose the right combination of fluorescent antibody conjugates for your immunophenotyping experiments. This includes fluorophore guides, articles, scientific posters, application notes, videos and more.
Antibody conjugates, antibody labeling, and fluorophores features
Selection guide
Fluorophore selection guide
These selection guides feature a dashboard for each fluorophore that provide the basic properties, allowing you to find the right fluorophore for your experiment.
Scientific poster
T lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes (PDF)
This scientific poster highlights the building of a 14-color flow cytometry panel using Invitrogen eBioscience Super Bright polymer dyes which represent a suite of bright fluorophores excited by the violet laser (405 nm).
Antibody conjugates, antibody labeling, and fluorophores learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2017) | Validated 10-color T cell panel using the Attune NxT Flow Cytometer | antibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry |
| BioProbes article (2018) | A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studies | antibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability |
| BioProbes article (2017) | Harness immune checkpoints to combat tumors—Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays | antibodies, cancer, flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC) |
| BioProbes article (2017) | Bright fluorescent polymer dyes for the violet laser—Super Bright antibody conjugates | antibodies, antibody labeling, Attune NxT, flow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry, Super Bright |
| BioProbes article (2017) | T lymphocyte immunophenotyping—14-color flow cytometry panel featuring Super Bright antibody conjugates | antibodies, Attune NxT, immunology, immunophenotyping, multicolor flow cytometry, Super Bright |
| BioProbes article (2014) | Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometry | cell proliferation, Click-iT, cell proliferation, multicolor flow cytometry |
| BioProbes article (2010) | Optimal pairing of dyes and antibodies for flow cytometry | antibodies, fluorescence, multicolor flow cytometry, particles Qdot |
| BioProbes article (2009) | Novel solid-phase antibody labeling | Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample preparation, Zenon |
| BioProbes article (2008) | Using Qdot nanocrystal primary antibody conjugates in flow cytometry | fluorescent dyes, multicolor flow cytometry, nanocrystals, Qdot, sample preparation, set up |
| BioProbes article (2007) | Quantum dots as replacements for tandem dyes in flow cytometry | fluorescent dyes, multicolor flow cytometry, nanocrystals, protein labeling, Qdot |
| BioProbes article (2007) | Simultaneously visualize surface and intracellular markers | antibody labeling, cell fixatives, flow cytometry, fluorescent dyes, sample preparation, Zenon |
| BioProbes article (2006) | A guide to antibody labeling and detection | Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nanocrystals, Qdot, Zenon |
| BioProbes article (2006) | Multicolor immunodetection using the violet laser | Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| Blog post | Super Bright conjugates for flow cytometry | antibodies, Attune NxT, flow cytometry, immunophenotyping, multicolor flow cytometry, Super Bright |
| Fluorophore | Pacific Blue dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Pacific Green dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Pacific Orange dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Super Bright 436 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 600 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 645 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 702 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Allophycocyanin (APC) | antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes |
| Fluorophore | R-phycoerythrin (R-PE) | antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes |
| Fluorophore | Cyan fluorescent protein (CFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Green fluorescent protein (GFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Red fluorescent protein (RFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Fluorophore selection guide | antibody conjugates, flow cytometry, immunophenotyping |
| Molecular Probes Handbook | Phycobiliproteins—Section 6.4 | antibody labeling, multicolor flow cytometry, tandem conjugates |
| Molecular Probes Handbook | Using the Fluorescence SpectraViewer—Note 23.1 | flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 | antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon |
| Scientific poster (2018) | Modular, defined assembly of antibody-based constructs using click chemistry | Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids |
| Scientific poster (2018) | Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia | antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research |
| Scientific Poster (2017) | T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes | antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright |
| Scientific poster (2014) | Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection | cell proliferation, Click-iT, flow cytometry, immunophenotyping |
| Scientific poster (2013) | Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping |
| Scientific poster (2008) | Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants | Alexa Fluor, antibodies, cell signaling, cells, flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon |
| Selection guide | Human CD & other cellular antigens | flow cytometry, immunology, immunophenotyping, extracellular staining |
| Selection guide | Mouse CD & other cellular antigens | flow cytometry, immunology, immunophenotyping, extracellular staining |
| How-to video | Tutorial: Labeling a purified antibody Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield. | antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| How-to video | How to site specifically label your antibody using SiteClick technology This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain. | antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection |
| Webinar | A practical approach to antibody labeling The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will:
| antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF) |
| Webinar | Learn to choose the right fluorophore when designing experiments The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore:
| antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.