Controls, compensation and calibration are all critical to obtaining accurate results in flow cytometric analysis. In this section you will find educational resources including application notes, videos, articles and other useful tools to help you set up your flow cytometer and analyze your samples.
Controls, compensation and calibration features
Webinar
AccuCheck ERF Reference Particles: Easy to Use Flow Cytometry Intensity Reference with Accurate NIST-Assigned Values
Scientific poster
Evaluation of multiple techniques for PMT optimization (PDF)
This study compares a variety of techniques and calculations for setting your flow cytometer photomultiplier tube and obtaining the minimum voltage required.
Controls, compensation and calibration learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2018) | High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates | Attune/Attune NxT Flow Cytometer, Autosampler |
| Application note (2015) | Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells | Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| BioProbes article (2018) | A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studies | antibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability |
| BioProbes article (2015) | Journal Club—Multiparameter detection of early apoptosis markers without compensation | apoptosis, Attune NxT, cell structure-mitochondria, flow cytometry compensation, fluorescence, immunophenotyping |
| BioProbes article (2015) | No-compensation immunophenotyping using a four-laser flow cytometer | Attune NxT, flow cytometer, fluorescence, immunophenotyping, multicolor flow cytometry |
| BioProbes article (2014) | Obtain the highest-quality data your system can deliver—Fluorescent microspheres for calibrating microscopes and flow cytometers | calibration, cell counting, flow cytometer, fluorescence microscopy/fluorescence imaging, microscope calibration, particles |
| BioProbes article (2009) | Accurate compensation is as easy as AbC | calibration, compensation, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles |
| Molecular Probes Handbook | Microspheres—Section 6.5 | flow cytometer calibration, fluorescence microscopy/fluorescence imaging, microscope calibration, particles |
| Molecular Probes Handbook | Flow cytometry reference standards—Section 23.2 | flow cytometer |
| Protocols | OneComp and UltraComp Compensation Beads protocols for flow cytometry (Invitrogen eBioscience reagents) | compensation, flow cytometry |
| Scientific poster (2018) | Maintaining even temperature distribution over chilled samples in 96-well plates and micro-centrifuge tubes | academic research solutions, microplates, quality control, temperature control |
| Scientific poster (2018) | A comparison of GFP BrightComp eBeads Compensation Bead Kit performance against multiple GFP variants | Attune/Attune NxT, beads, flow cytometry compensation, fluorescent protein detection, |
| Scientific poster (2018) | Evaluation of multiple techniques for PMT optimization | Attune/Attune NxT Flow Cytometer, flow cytometer calibration, photomultiplier settings |
| Scientific Poster (2016) | A microsphere-based universal compensation approach | Attune NxT, Flow cytometer calibration, flow cytometry sample preparation |
| Scientific poster (2010) | Use of surface-modified microspheres for setting compensation in multicolor flow cytometry | antibodies, cells, flow cytometry compensation, fluorescent dyes, multicolor flow cytometry, particles, violet laser-excited reagents |
| Tutorial video | Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data | flow cytometer calibration, flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Flow Cytometry | flow cytometer calibration, multicolor flow cytometry |
| Webinar | Basics of flow cytometry, Part II: Compensation This presentation provides an overview of basic fluorochromes used in flow cytometry. Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation. | flow cytometry data, flow cytometry set up, multicolor flow cytometry |
| Webinar | Basics of multi color flow cytometry panel design With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
| antibodies, compensation, multicolor flow cytometry |
| Webinar | AccuCheck ERF Reference Particles: Easy to Use Flow Cytometry Intensity Reference with Accurate NIST-Assigned Values | AccuCheck ERF Reference Particles, calibration and size reference beads, controls, standards and compensation |
| Scientific Poster CYTO 2022 | Fluorescent nanoparticle flow cytometry calibrators with NIST-assigned ERF and concentration values for viral and EV analysis | AccuCheck ERF Reference Particles, calibration and sizereference beads, controls, standards and compensation |
| Scientific Poster CYTO 2022 | Internally stained multiple fluorescent microsphere intensity reference with NIST-assigned ERF values for quantitative flow cytometry analysis | AccuCheck ERF Reference Particles, calibration and sizereference beads, controls, standards and compensation |