Flow cytometry is used to obtain quantifiable results in reference to the physical characteristics of single cells. Microspheres are small, non-biological beads that can be used as a (1) standard for flow cytometer instrument calibration, (2) reference for cell or particle size, and/or (3) experimental control for fluorescence emission spillover.
These beads are designed to provide:
- Consistent instrument performance with minimal data variation for alignment, size calibration, and sorting set-up
- Convenience to save sample, and set compensation for antibody, reagent, and fluorescent proteins
- Comparable data between experiments and instruments by setting a standard