Precise multiplex gene expression in high throughput
Invitrogen QuantiGene Plex assays offer a fast and high-throughput solution for multiplex gene expression quantitation, allowing the simultaneous measurement of up to 80 genes of interest in a single well of a 96- or 384-well plate using any Luminex instrument for data acquisition.
About QuantiGene Plex assays
The QuantiGene Plex assay is hybridization-based and incorporates branched DNA (bDNA) technology, which uses signal amplification rather than target amplification for direct measurement of RNA transcripts. The assay is extremely easy to use with a simple ELISA-like workflow, and it does not require RNA purification and reverse transcription. There is an excellent correlation for data acquired via the gold standard qPCR and QuantiGene.
Unique benefits
Works with difficult sample types and no RNA isolation
In addition to purified RNA, lysates, and homogenates, this assay works with degraded and cross-linked RNA in FFPE tissues and with whole blood; no RNA purification required
True multiplexing
Measure up to 80 genes of interest, including housekeeping genes, in the same well with no cross-reactivity while utilizing small sample volumes
Standardized platform and correlation with qPCR
96-well plate format compatible with Luminex 200, and FLEXMAP 3D systems and 384-well with FLEXMAP 3D and xMAP INTELLIFLEX systems; excellent correlation with gold standard qPCR
Simple workflow
ELISA-like workflow for target hybridization and signal amplification with no RNA purification or reverse transcription needed
Large inventory of validated genes and panels
Select from over 22,000 genes to create pathway and disease-themed panels or choose one of hundreds of preconfigured panels
Customization
Custom panels can be created within 3–4 weeks
Detect low-abundance genes
Exquisite sensitivity allows for the basal measurement of low-expression genes
Complementary and highly correlative
Matched ProcartaPlex protein panels are available
Flexible formats
A variety of formats for different research needs.
QuantiGene Gene Expression Assay Ordering
Three product kits create the QuantiGene Plex gene expression assay. All QuantiGene Plex panels contain a target-specific probe set and magnetic capture beads. There are over 22,000 validated gene targets to choose from, but if you cannot find your target of interest, it can be added to your custom panel for a small design fee. We functionally verify every new panel, and custom panels are designed, built, validated, and shipped within 3–4 weeks of your order. It is recommended that every panel include 3–5 housekeeping genes to enable data normalization using a geometric mean.
| QuantiGene Plex Panel | QuantiGene Sample Processing kit (Optional) | QuantiGene Assay kit |
Panel options and features
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Kits available for
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Reagents, plates, and seals for |
Specifications
| Limit of detection | ≤1,000–2,000 transcripts/assay well |
| Limit of quantitation | ≤2,000–4,000 transcripts/assay well |
| Linear dynamic range | ≥3 logarithmic units |
| Assay CV | ≤15% intra-assay; ≤20% inter-assay |
| Compatible sample types | Cultured cells, bacteria, whole blood, PAXgene blood, Invitrogen Tempus samples, dried blood spots, fresh-frozen tissues (animal or plant), FFPE samples, purified RNA |
| Assay format | 96- or 384-well plate |
| Targets/well | 3–80 |
Learn more
Learn about the QuantiGene Plex Assay workflow, browse through various resources, see sample data, and more!
For Research Use Only. Not for use in diagnostic procedures.