Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Find step-by-step instructions for your imaging experiments. If you are just starting, we recommend the Molecular Probes School of Fluorescence as a starting point. Protocols linked below are to be used as a guide only, and may require optimization for your experiment.
Cell proliferation protocols for imaging
- BrdU Labeling and Detection Protocol
- Click-iT EdU HCS Assays Protocol
- Click-iT EdU Imaging Kits Protocol
- Click-iT Plus EdU Imaging Kits Protocol
- Click-iT EdU and Click-iT Plus EdU for In Vivo Protocol
Cell viability protocols for imaging
- Image-iT DEAD Green Kit Protocol
- LIVE BacLight Bacterial Gram Stain Kit Protocol
- LIVE/DEAD BacLight Bacterial Viability Kit Protocol
- LIVE/DEAD Cell Imaging Kit (488/570) Protocol
- LIVE/DEAD Sperm Viability Kit Imaging Protocol
- LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells Protocol
- LIVE/DEAD Yeast Viability Kit Protocol
- NucGreen Dead 488 ReadyProbes Reagent Protocol for Viability
- NucRed Dead 647 ReadyProbes Reagent Protocol for Viability
- ReadyProbes Cell Viability Imaging Kit, Blue/Green Protocol
- ReadyProbes Cell Viability Imaging Kit, Blue/Red Protocol
Cell structure protocols for imaging
- ActinGreen 488 ReadyProbes Reagent Protocol
- ActinRed 555 ReadyProbes Reagent Protocol
- DAPI Protocol for Fluorescence Imaging
- F-Actin Labeling with Fluorescent Phalloidin Protocol
- Hoechst 33342 Protocol for Imaging
- Functional Mitochondrial Staining with TMRM Protocol
- NucBlue Fixed Cell ReadyProbes Reagent Protocol
- NucBlue Live ReadyProbes Reagent Protocol
- NucGreen Dead 488 ReadyProbes Reagent Protocol for Fixed Cells
- NucRed Dead 647 ReadyProbes Reagent Protocol for Fixed Cells
- NucRed Live 647 ReadyProbes Reagent Protocol
- SYTO 59 Nuclear Staining Protocol
- SYTO 82 Nuclear Staining Protocol
- SYTOX Green Nucleic Acid Stain Protocol
- TO-PRO-3 Stain Protocol
General antibody staining protocols
- Automated Multiplex IHC Staining with Aluora Spatial Amplification Kits and Leica BOND
- Immunofluorescent Staining of Intracellular Antigens on Cultured Cells (Invitrogen eBioscience reagents)
- IHC Frozen Tissue—Direct Method (Invitrogen eBioscience reagents)
- IHC Frozen Tissue—Indirect Method (purified) (Invitrogen eBioscience reagents)
- IHC Frozen Tissue—Indirect Method (biotin) (Invitrogen eBioscience reagents)
- IHC FFPE Tissue Trypsin Digestion—Direct Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue Trypsin Digestion Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue Low pH Antigen Retrieval—Direct Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue Low pH Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue High pH Antigen Retrieval—Direct Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue High pH Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
- IHC Staining of Formalin-Fixed Paraffin-Embedded Tissues (Invitrogen eBioscience reagents)
- Colorimetric FFPE—High pH Antigen Retrieval (Invitrogen eBioscience reagents)
- Colorimetric FFPE—Low pH Antigen Retrieval (Invitrogen eBioscience reagents)
- Colorimetric FFPE—Trypsin Digestion (Invitrogen eBioscience reagents)
- ICC Formaldehyde Fixed Cells—Direct Method (Invitrogen eBioscience reagents)
- ICC Formaldehyde Fixed Cells—Indirect Method (Invitrogen eBioscience reagents)
- ICC Formaldehyde Fixed, Permeabilized Cells—Direct Method (Invitrogen eBioscience reagents)
- ICC Formaldehyde Fixed, Permeabilized Cells—Indirect Method (Invitrogen eBioscience reagents)
- ICC Methanol Fixed Cells—Direct Method (Invitrogen eBioscience reagents)
- ICC Methanol Fixed Cells—Indirect Method (Invitrogen eBioscience reagents)
- ICC Unfixed Cells—Direct Method (Invitrogen eBioscience reagents)
- Mounting Coverslips Protocol
- Simple Immunolabeling with Primary and Secondary Antibodies Protocol
- Tyramide staining for tissue samples
- 3D Organoid Staining
- ViewRNA tissue fluorescence assay protocol
- ViewRNA tissue colorimetric assay protocol
For Research Use Only. Not for use in diagnostic procedures.