Antibodies for Detecting Membrane-Surface Labels—Note 13.2

For detecting labels at the membrane surface, we offer antibodies that recognize the following labels:

• Alexa Fluor 488 dye (A11094)
• BODIPY FL dye (A5770)
• Alexa Fluor 405 and Cascade Blue dyes (A5760)
• Dansyl (A6398)
• Dinitrophenyl chromophore (A6423, A6430, A6435, A11097, Q17421MP)
• Fluorescein and Oregon Green dyes (A889, A982, A6413, A6421, A11090, A11091, A11095, A11096, Q15421MP, Q15431MP)
• Green Fluorescent Protein (GFP, A6455, A10259, A10262, A10263, A11120, A11121, A11122, A21311, A21312, A31851, A31852, G10362)
• Lucifer yellow (A5750, A5751)
• Tetramethylrhodamine (A6397)
• Texas Red dye (A6399)

Fluorescent conjugates of several of these anti-dye and anti-hapten antibodies are available; see Anti-Dye and Anti-Hapten Antibodies—Section 7.4 and Anti-fluorophore antibodies and their conjugates—Table 7.8 for complete product information. These antibodies can be used for direct detection of labeled phospholipids via fluorescence quenching (or fluorescence enhancement, in the case of the anti-dansyl antibody). When used in conjunction with phospholipids with dye-labeled head groups (Phospholipids with labeled head groups—Table 13.1), they are important tools for:

• Studies of molecular recognition mechanisms at membrane surfaces
• Lipid diffusion measurements
• Quantitation of lipid internalization by endocytosis

In addition to anti-fluorophore antibodies, we offer a selection of streptavidin conjugates (Avidin, Streptavidin, NeutrAvidin and CaptAvidin Biotin-Binding Proteins and Affinity Matrices—Section 7.6, Molecular Probes avidin, streptavidin, NeutrAvidin and CaptAvidin conjugates—Table 7.9) for detecting biotinylated phospholipids.