Download: Protein Gel Guide
Pour your own polyacrylamide (SDS-PAGE) mini or midi gels with our sealed empty gel cassettes. These cassettes give you the option of hand casting your own gels while enjoying the benefits of the Mini Gel Tank and SureLock systems.
 Bolt Mini Gel Cassettes |  Novex Empty Cassettes | |
| Size available | Mini Gel: 8x8cm (1.0 mm thick) | Mini gel: 8x8 cm (1.5 or 1.0 mm thick) Midi gel: 8x13 cm (1.0 mm thick) |
| Equipment required | Mini Gel Tank or XCell SureLock Mini-Cell | Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only) |
| Combs available | 10 and 12-well Wedgewell (high capacity sample well) | 1, 2, 2D, 5, 10, 12, 15-well |
| Product benefits |
|
|
Recommended maximum loading volumes
| Well Type | 1.0 mm thickness | 1.5 mm thickness |
|---|---|---|
| 10-well Wedgewell | 60 μL | |
| 12-well Wedgewell | 45 μL | |
| 1-well | 700 μL | |
| 5-well | 60 μL | |
| 10-well | 25 µL | 37 µL |
| 12-well | 20 µL | |
| 15-well | 15 µL | 25 µL |
| 2D-well | 400 µL | |
| 12+2* well | 45 µL + 15 µL | |
| 20* well | 25 µL | |
| 26* well | 15 µL |
* Midi Gel
Documents
Gel casting recipes
Choose the polyacrylamide percentage and type of well for your application based upon the expected molecular weight of your protein of interest and the volume of your sample.
Resolve large molecules with low percentage gels and small molecules with high percentage gels. If the molecular weight of the molecule is unknown, or the sample contains a wide range of molecules, use a mid-range percentage gel.
Recipes for SureCast reagents
Prepare resolving gel solution according to the volumes in the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.
Note: Solutions do not require degassing.
| Polyacrylamide % | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Solution | 4% | 6% | 8% | 10% | 12% | 14% | 16% | 18% | 20% |
| SureCast Acrylamide (40%) | 0.8 mL | 1.2 mL | 1.6 mL | 2.0 mL | 2.4 mL | 2.8 mL | 3.3 mL | 3.6 mL | 4.0 mL |
| SureCast Resolving Buffer | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL |
| Distilled water | 5.1 mL | 4.7 mL | 4.3 mL | 3.9 mL | 3.5 mL | 3.1 mL | 2.7 mL | 2.3 mL | 1.9 mL |
| 10% SureCast APS | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL |
| SureCast TEMED** | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL |
** Add this last and mix well just before the gel is to be poured
Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.
| Solution | 4% |
|---|---|
| SureCast Acrylamide (40%) | 0.30 mL |
| SureCast Stacking Buffer | 0.75 mL |
| Distilled water | 1.92 mL |
| 10% SureCast APS | 30 µL |
| SureCast TEMED** | 3 µL |
Recipes for standalone reagents
Prepare the following stock solutions: All solutions can be stored at room temperature.
50% Acrylamide/BIS (29:1)
Store up to two months in a dark glass bottle | Separating Gel Buffer (1 M Tris-HCl, pH 8.8)
| Stacking Gel Buffer (0.375M Tris HCl, pH 6.8)
| Catalyst- Ammonium Persulfate (Make fresh the day of use)
|
10% SDS
| 50% Sucrose
|
Separating gels
The following recipes are for approximately 25 mL of separating gels, enough for four 1.0-mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast.
| Solution | 6% Gel | 8% Gel | 10% Gel | 12% Gel | 14% Gel | 16% Gel | 18% Gel | 20% Gel |
|---|---|---|---|---|---|---|---|---|
| 50% Acrylamide/BIS | 3.0 mL | 4.0 mL | 5.0 mL | 6.0 mL | 7.0 mL | 8.0 mL | 9.0 mL | 10.0 mL |
| Separating Gel Buffer | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL |
| 10% SDS | 250 µL | 250 µL | 250 µL | 250 µL | 250 µL | 250 µL | 250 µL | 250 µL |
| 50% Sucrose* | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL |
| Water | 7.8 mL | 6.8 mL | 5.8 mL | 4.8 mL | 3.7 mL | 2.7 mL | 1.7 mL | 750 µL |
| TEMED** | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL |
| Catalyst** | 625 µL | 625 µL | 625 µL | 625 µL | 625 µL | 625 µL | 625 µL | 625 µL |
** Add these last and mix well just before the gel is to be poured.
Stacking gels
Following recipe is for 4% stacking gels (12.5 mL)
| Solution | 4% |
|---|---|
| 50% Acrylamide/BIS | 1.0 mL |
| Stacking Gel Buffer | 4.2 mL |
| 10% SDS | 125 µL |
| Water | 6.3 mL |
| TEMED** | 5.0 µL |
| Catalyst** | 1.0 mL |
** Add this last and mix well just before the gel is to be poured
Bolt Mini Gel Cassettes | Novex Empty Cassettes | |
| Size available | Mini Gel: 8x8cm (1.0 mm thick) | Mini gel: 8x8 cm (1.5 or 1.0 mm thick) Midi gel: 8x13 cm (1.0 mm thick) |
| Equipment required | Mini Gel Tank or XCell SureLock Mini-Cell | Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only) |
| Combs available | 10 and 12-well Wedgewell (high capacity sample well) | 1, 2, 2D, 5, 10, 12, 15-well |
| Product benefits |
|
|
Recommended maximum loading volumes
| Well Type | 1.0 mm thickness | 1.5 mm thickness |
|---|---|---|
| 10-well Wedgewell | 60 μL | |
| 12-well Wedgewell | 45 μL | |
| 1-well | 700 μL | |
| 5-well | 60 μL | |
| 10-well | 25 µL | 37 µL |
| 12-well | 20 µL | |
| 15-well | 15 µL | 25 µL |
| 2D-well | 400 µL | |
| 12+2* well | 45 µL + 15 µL | |
| 20* well | 25 µL | |
| 26* well | 15 µL |
* Midi Gel
Documents
Gel casting recipes
Choose the polyacrylamide percentage and type of well for your application based upon the expected molecular weight of your protein of interest and the volume of your sample.
Resolve large molecules with low percentage gels and small molecules with high percentage gels. If the molecular weight of the molecule is unknown, or the sample contains a wide range of molecules, use a mid-range percentage gel.
Recipes for SureCast reagents
Prepare resolving gel solution according to the volumes in the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.
Note: Solutions do not require degassing.
| Polyacrylamide % | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Solution | 4% | 6% | 8% | 10% | 12% | 14% | 16% | 18% | 20% |
| SureCast Acrylamide (40%) | 0.8 mL | 1.2 mL | 1.6 mL | 2.0 mL | 2.4 mL | 2.8 mL | 3.3 mL | 3.6 mL | 4.0 mL |
| SureCast Resolving Buffer | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL | 2.0 mL |
| Distilled water | 5.1 mL | 4.7 mL | 4.3 mL | 3.9 mL | 3.5 mL | 3.1 mL | 2.7 mL | 2.3 mL | 1.9 mL |
| 10% SureCast APS | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL | 80 µL |
| SureCast TEMED** | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL | 8 µL |
** Add this last and mix well just before the gel is to be poured
Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.
| Solution | 4% |
|---|---|
| SureCast Acrylamide (40%) | 0.30 mL |
| SureCast Stacking Buffer | 0.75 mL |
| Distilled water | 1.92 mL |
| 10% SureCast APS | 30 µL |
| SureCast TEMED** | 3 µL |
Recipes for standalone reagents
Prepare the following stock solutions: All solutions can be stored at room temperature.
50% Acrylamide/BIS (29:1)
Store up to two months in a dark glass bottle | Separating Gel Buffer (1 M Tris-HCl, pH 8.8)
| Stacking Gel Buffer (0.375M Tris HCl, pH 6.8)
| Catalyst- Ammonium Persulfate (Make fresh the day of use)
|
10% SDS
| 50% Sucrose
|
Separating gels
The following recipes are for approximately 25 mL of separating gels, enough for four 1.0-mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast.
| Solution | 6% Gel | 8% Gel | 10% Gel | 12% Gel | 14% Gel | 16% Gel | 18% Gel | 20% Gel |
|---|---|---|---|---|---|---|---|---|
| 50% Acrylamide/BIS | 3.0 mL | 4.0 mL | 5.0 mL | 6.0 mL | 7.0 mL | 8.0 mL | 9.0 mL | 10.0 mL |
| Separating Gel Buffer | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL | 9.4 mL |
| 10% SDS | 250 µL | 250 µL | 250 µL | 250 µL | 250 µL | 250 µL | 250 µL | 250 µL |
| 50% Sucrose* | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL | 4.0 mL |
| Water | 7.8 mL | 6.8 mL | 5.8 mL | 4.8 mL | 3.7 mL | 2.7 mL | 1.7 mL | 750 µL |
| TEMED** | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL | 6.25 µL |
| Catalyst** | 625 µL | 625 µL | 625 µL | 625 µL | 625 µL | 625 µL | 625 µL | 625 µL |
** Add these last and mix well just before the gel is to be poured.
Stacking gels
Following recipe is for 4% stacking gels (12.5 mL)
| Solution | 4% |
|---|---|
| 50% Acrylamide/BIS | 1.0 mL |
| Stacking Gel Buffer | 4.2 mL |
| 10% SDS | 125 µL |
| Water | 6.3 mL |
| TEMED** | 5.0 µL |
| Catalyst** | 1.0 mL |
** Add this last and mix well just before the gel is to be poured
Resources
For Research Use Only. Not for use in diagnostic procedures.