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| NanoDrop One/Onec | NanoDrop Eight | Multiskan SkyHigh | Varioskan LUX |
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What is the lower detection limit (dsDNA)? | - Pedestal: 2.0 ng/µL
- Cuvette: 0.2 ng/µL
| 2.0 ng/µL | Depends on sample format and volume used (in the case of microplates)* See details- Cuvette: 0.45 ng/µL
- µDrop Plate: 9 ng/µL
Microplates: 96 well plates (normal)
- 70 µL (minimum) 2.1 ng/µL
- 140 µL (good) 1.1 ng/µL
- 370 µL (maximal) 0.4 ng/µL
96 well plates (half-area)
- 35 µL (minimum) 2.1 ng/µL
- 60 µL (good) 1.2 ng/µL
- 180 µL (maximal) 0.5 ng/µL
384 well plates
- 20 µL (minimum) 2.5 ng/µL
- 40 µL (good) 1.3 ng/µL
- 120 µL (maximal) 0.5 ng/µL
| Depends on sample format and volume used (in the case of microplates)* See details- Cuvette: 0.15 ng/µL
- µDrop Plate: 3 ng/µL
Microplates: 96 well plates (normal)
- 70 µL (minimum) 0.7 ng/µL
- 140 µL (good) 0.4 ng/µL
- 370 µL (maximal) 0.5 ng/µL
96 well plates (half-area)
- 35 µL (minimum) 0.7 ng/µL
- 60 µL (good) 0.4 ng/µL
- 180 µL (maximal) 0.1 ng/µL
384 well plates
- 20 µL (minimum) 0.8 ng/µL
- 40 µL (good) 0.4 ng/µL
- 120 µL (maximal) 0.2 ng/µL
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What is the upper detection limit (dsDNA)? | - Pedestal: 27 500 ng/µL
- Cuvette: 75 ng/µL
| 10000 ng/µL | Depends on sample format and volume used (in the case of microplates) See details- Cuvette: 125 ng/µL
- µDrop Plate: 2500 ng/µL
Microplates: 96 well plates (normal)
- 70 µL (minimum) 590 ng/µL
- 140 µL (good) 310 ng/µL
- 370 µL (maximal) 120 ng/µL
96 well plates (half-area)
- 35 µL (minimum) 560 ng/µL
- 60 µL (good) 320 ng/µL
- 180 µL (maximal) 110 ng/µL
384 well plates
- 20 µL (minimum) 690 ng/µL
- 40 µL (good) 350 ng/µL
- 120 µL (maximal) 120 ng/µL
| Depends on sample format and volume used (in the case of microplates) See details- Cuvette: 200 ng/µL
- µDrop Plate: 4000 ng/µL
Microplates: 96 well plates (normal)
- 70 µL (minimum) 950 ng/µL
- 140 µL (good) 500 ng/µL
- 370 µL (maximal) 190 ng/µL
96 well plates (half-area)
- 35 µL (minimum) 900 ng/µL
- 60 µL (good) 520 ng/µL
- 180 µL (maximal) 190 g/µL
384 well plates
- 20 µL (minimum) 1100 ng/µL
- 40 µL (good) 570 ng/µL
- 120 µL (maximal) 200 ng/µL
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What are the available sample formats? | | Drop (1 µL) | - 96-384 well plates
- µDrop Plate (2-10 µL)
- cuvettes (not all models)
| - 6-384 well plates
- µDrop Plate (2-10 µL)
- cuvettes (not all models)
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How many samples can be measured at a time? (throughput) | One sample at a time | Up to 8 | Dependent on assay format See details- one sample at a time with cuvettes
- up to 16 with µDrop plate
- up to 96 with 96-well plates
- up to 384 with 384 well plates
| Dependent on assay format See details- one sample at a time with cuvettes
- up to 16 with µDrop plate
- up to 96 with 96-well plates
- up to 384 with 384 well plates
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What volume of sample is required to measure? | 1 µL | 1 µL | As little as 2 µL depending on assay format See details- 2-10 µL with µDrop Plate (smallest volume required)
- 70-300 µL with 96 well plates (normal)
- 20-100 µL with 384 well plates
- 1 mL with 10x10 mm cuvettes (70 µL for microcuvettes)
| As little as 2 µL depending on assay format See details- 2-10 µL with µDrop Plate (smallest volume required)
- 70-300 µL with 96 well plates (normal)
- 20-100 µL with 384 well plates
- 1 mL with 10x10 mm cuvettes (70 µL for microcuvettes)
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How much time it takes to measure one sample? | 8 seconds | < 20 seconds for 8 samples | Around 1 minute for 16 microvolume samples in the µDrop Plate See detailsWhen performed with the user interface touch screen or SkanIt software (fast mode) | Around 1.3 minutes for 16 microvolume samples in the µDrop Plate See detailsWhen performed with the user interface touch screen or SkanIt software (fast mode) |
Does it provide spectral data of the sample? | Yes | Yes | Yes | Yes |
Does it give information on sample purity? | Yes See detailsIt provides full spectral data (A260/A280 and A260/A230 ratios) as well as Acclaro Contaminant Analysis | Yes See detailsIt provides full spectral data (A260/A280 and A260/A230 ratios) as well as Acclaro Contaminant Analysis | Yes See detailsIt provides full spectral data (A260/A280 and A260/A230 ratios) | Yes See detailsIt provides full spectral data (A260/A280 and A260/A230 ratios) |
Does it detect specific contaminants in the sample? | Yes See detailsAcclaro Sample Intelligence technology detects protein, phenol and guanidine salts and it gives true concentration of the nucleic acids | Yes See detailsAcclaro Sample Intelligence technology detects protein, phenol and guanidine salts and it gives true concentration of the nucleic acids | No See detailsSome information is available, based on the purity ratios | No See detailsSome information is available, based on the purity ratios |
Does it distinguish between DNA and RNA in the sample? | Yes See detailsDistinguishes between mammalian DNA and mammalian RNA | Yes See detailsDistinguishes between mammalian DNA and mammalian RNA | No | No |
Does it support oligonucleotide quantification? | Yes See detailsWith embedded applications and custom methods | Yes See detailsWith embedded applications and custom methods | Yes | Yes |
Does it provide pre-configured protocols? | Yes See detailsThere are user interface touch screen and custom-protocols | Yes See detailsThere are user interface touch screen and custom-protocols | Yes See detailsThere are pre-configured protocols in the interface touch screen and SkanIt software Cloud Library, free with SkanIt Software | Yes See detailsThere are pre-configured protocols in the SkanIt software Cloud Library, free with SkanIt Software |
Does it connect to the Thermo Fisher Connect? | Yes | No | Yes | No |
Does it provide 21CFR Part 11 compliance? | Yes, with optional software | Yes, with optional software | Yes See detailswith SkanIt software Drug Discovery Edition | Yes See detailswith SkanIt software Drug Discovery Edition |
Does it offer robotic automation compatibility? | No | No | Yes | Yes |
How is the instrument operated? | Via a touch screen user interface or via the NanoDrop One PC control software | With a PC software (Nanodrop Eight Operating software) | Via a touch screen user interface or the PC software (SkanIt software) | Via a PC software (SkanIt software) |
What is the overall ease of use? | ++++ | ++++ | ++++ | +++ |
What is the basic protocol? | See details- Pipette buffer on pedestal and blank instrument
- Pipette sample directly on pedestal
- Follow instructions on touchscreen to run protocol
- Results are available within seconds
| See details- Pipette buffer on all 8 pedestals and blank instrument
- Pipette samples directly on 8 pedestals
- Run protocol using PC software
- Results are available within seconds
| See details- Pipette blank(s) and samples in microplates or µDrop plate
- Choose how you want to run your assay:
- Choose the pre-configured assay using the touch screen
- Choose the pre-built assay in the SkanIt Cloud Library within SkanIt Software
- Set up the assay using SkanIt Software
- Results are available quickly
| See details- Pipette blank(s) and samples into microplates or µDrop plate
- Either choose the pre-built protocol in the SkanIt Cloud Library or set-up the assay with SkanIt Software
- Results are available quickly
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What is the instrument price category? | Medium | Medium | Medium | High |
What are the ownership costs? | No recurring costs | No recurring costs | If using µDrop Plate, no recurring costs; otherwise UV-compatible clear plates | If using µDrop Plate, no recurring costs; otherwise UV-compatible clear plates |
* Theoretical limit of detection (LOD), estimated according to IUPAC, based on the precision of the blanks (3*SD), from the precision specifications of Multiskan SkyHigh and Varioskan LUX microplate readers. |