Start your scale-up with a T75 flask that has the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes straight-neck or angled-neck options and surface treatment alternatives that help ensure your healthy tissue cells expand and grow in the flask of your choice.
Some useful numbers such as surface area and volumes of dissociation solutions are given below for T75 flasks.
Please note: The number of cells on a flask will vary with cell type. For this table, 2.1 x 106 HeLa cells were seeded and the confluent flask contained 8.4 x 106 cells.
| T75 flasks have a surface area of 75 cm2 | ||
|---|---|---|
| Dissociation reagent | Volume of reagent | Volume of growth medium |
| TrypLE Express enzyme | 5.0 mL | 8–15 mL |
| TrypLE Select (1x) enzyme | 5.0 mL | |
| TrypLE Select (10x) enzyme | 0.3 mL diluted to 5.0mL in DPBS/1 mM EDTA | |
| Versene reagent | 5.0 mL | |
| Trypsin | 5.0 mL | |
Ergonomically designed for maximal access to the growth surface, this low-profile flask optimizes incubator space and features angled walls for complete viewing under microscopes. Additional features include multiple cap options, and sterilization by gamma irradiation with non-pyrogenic certification.
To help ensure flexible, reproducible, and reliable results across every stage of cell culture, T75 flasks come in a range of formats, sizes, and surfaces. Please see below for guidance on product selection for specific cell types and desired cell culture ranges.
Made with high-quality, optically clear virgin polystyrene, the Thermo Scientific Nunc non-treated flasks, with high-level sterility assurance, are ideal for suspension culture of non-adherent cells.
Composed of cell culture-treated polystyrene and surface treated with proprietary Nunclon Delta surface, these T75 flasks support optimal growth and attachment of cells to the growth surface.
Filtered caps allow for constant airflow while vent/close caps allow for sufficient gas exchange.
This ultra-low binding surface is designed to grow scaffold-free spheroid and organoid cultures and is ideally suited for cancer and stem cells.
Thermo Scientific BioLite cell culture treated flasks with filter cap and seal cap options suit a multitude of experimental needs while providing cost efficiency for a wide selection of applications in your cell culture routine.
Caps come in both vented and plug seal varieties.
Poly-D-lysine- (PDL) and collagen I-coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface.
PDL is an animal-free synthetic surface for adherent cultures or primary cells that are finicky and hard to attach, while collagen I, also excellent for finicky cells, is of animal origin. Selection of one surface over another depends on tissue type. Please review previous references for your specific cell line or, if necessary, test both.
Made with high-quality, optically clear virgin polystyrene, the Thermo Scientific Nunc non-treated flasks, with high-level sterility assurance, are ideal for suspension culture of non-adherent cells.
Composed of cell culture-treated polystyrene and surface treated with proprietary Nunclon Delta surface, these T75 flasks support optimal growth and attachment of cells to the growth surface.
Filtered caps allow for constant airflow while vent/close caps allow for sufficient gas exchange.
This ultra-low binding surface is designed to grow scaffold-free spheroid and organoid cultures and is ideally suited for cancer and stem cells.
Thermo Scientific BioLite cell culture treated flasks with filter cap and seal cap options suit a multitude of experimental needs while providing cost efficiency for a wide selection of applications in your cell culture routine.
Caps come in both vented and plug seal varieties.
Poly-D-lysine- (PDL) and collagen I-coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface.
PDL is an animal-free synthetic surface for adherent cultures or primary cells that are finicky and hard to attach, while collagen I, also excellent for finicky cells, is of animal origin. Selection of one surface over another depends on tissue type. Please review previous references for your specific cell line or, if necessary, test both.
Surface selection: Achieving optimal cell growth and harvesting
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
Establishing a dynamic micro-gravity spheroid 3D culture system
Learn about how to build specific 3D cell spheroid models and platforms to answer the pressing and relevant questions in cancer research.
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.
Surface selection: Achieving optimal cell growth and harvesting
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
Establishing a dynamic micro-gravity spheroid 3D culture system
Learn about how to build specific 3D cell spheroid models and platforms to answer the pressing and relevant questions in cancer research.
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.
For Research Use Only. Not for use in diagnostic procedures.
