Which human monocyte cell isolation kit is right for you?
| Highest purity for molecular assays | Affordable & pure, flow-compatible cells | Untouched—most physiological relevant cells | |||
|---|---|---|---|---|---|
| Isolation method | Depletion or positive isolation | Positive isolation | Negative isolation | ||
| Starting samples | Any sample (whole blood, MNC, bone marrow) | Any sample (whole blood, MNC, bone marrow) | MNC* | ||
| # MNC processed (mL whole blood processed) | 1-2 x 109 MNC 200 mL whole blood | 1-2 x 109 MNC 80 mL whole blood | 1 x 109 cells | ||
| Purity | Highest 98–100% | Higher 95–99% | High 90–95% | ||
| Yield | Highest 95–100% | Standard 45–60% | Standard 50–70% | ||
| Viability | >95% | >95% | >95% | ||
| Isolation time | ~30 min | ~60 min | ~50 min | ||
| Isolation temp. | Cold | Cold | Cold | ||
| Applications | Depletion of unwanted monocytes or positive isolation for molecular isolation of proteins & nucleic acids | Cellular assays (cell culture, cell activation/proliferation, cytokine studies) Molecular isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells) | ||
| Bead-free target cells | No | Yes (but antibody present) | Yes | ||
| Compatible with flow cytometry/sorting | No | Yes | Yes | ||
| Shelf life from production date | 3 years | 2 years | 2 years | ||
| Required materials | Magnets | Magnets | Magnets | ||
| Order now | Order now | Order now |
Learn more about our monocyte isolation kits
Dynabeads CD14
Dynabeads CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads CD14:
- Fast and efficient isolation of CD14+ cells from any sample—no columns required
- High purity, yield, and viability of positively isolated CD14+ cells for molecular downstream assays
- Fast and efficient depletion of CD14+ cells from any sample
Dynabeads FlowComp Human CD14 Kit
Dynabeads FlowComp Human CD14 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD14+ monocytes from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp Release Buffer.
- Rapid isolation of CD14+ monocytes from any sample—no columns required
- Pure and bead-free CD14+ monocytes for any downstream application
Dynabeads Untouched Human Monocytes Kit
The Dynabeads Untouched Human Monocytes Kit is used to isolate pure and viable monocytes from PBMC by negative isolation. The kit depletes T cells, B cells, NK cells, dendritic cells, granulocytes, and erythrocytes, while the negatively isolated human monocytes are left in the sample. The isolated monocytes cells are in perfect shape for any functional assay and application. Advantages of the Dynabeads Untouched Human Monocytes Kit:
- High purity, recovery, and viability of human monocytes
- Easy to use and to scale up
- No columns required
THIS IS FOR HUMAN RESEARCH—NOT FOR MOUSE