Maintain high level of cell viability:
Without supplementation with FBS or TPB (Tryptose Phosphate Broth) CD BHK-21 Production Medium supports suspension BHK-21 cells longer than GMEM supported both by TPB as well as 10% FBS. Cell viabilities started to decline after 24 hours in GMEM, whereas they were stable for four days in CD BHK-21 Production Medium.
Streamline the challenges of vaccine production
Vaccine production is complex. Manufacturers face strict regulatory controls during the development process while also anticipating global demand and the eventual production challenges of quantity and quality. Time-to-market and cost effectiveness are constant, critical considerations. Finding high performance media, tools and support are critical success factors in accelerating vaccine development and streamlining manufacturing efforts.
Our bioproduction products and services are here to support you in these challenges with performance Gibco media, advanced technology tools and knowledgeable expert support to help optimize your workflow, minimize risk and improve cost effectiveness.
What’s new: Gibco Residual TrypLE Enzyme ELISA Kit
Introducing the Residual TrypLE Enzyme ELISA Kit, a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) kit for the quantitative detection of the recombinant Gibco TrypLE enzyme.
Key features of the Residual TrypLE Enzyme ELISA Kit:
- High specificity—The antibodies are tailored for the TrypLE enzyme to enable unmatched specificity
- Rapid results—Results in just 1.5 hours helps boost your workflow efficiency
- Strong sensitivity—Detection as low as 0.032 ng/mL enables precise TrypLE enzyme assessment
- Compatibility—Works seamlessly with all forms and concentrations of TrypLE enzyme
- Reliability—Lot-to-lot consistency provided for reproducible results, helping establish trust in experiments
Performance serum-free media
Gibco performance serum-free media (SFM) and animal origin-free (AOF) media deliver superior cell growth and virus production to serum-supplemented systems while maximizing consistency and reliability and simplifying downstream purification.
Gibco CD BHK-21 Production Medium is the first cell culture medium supporting complete serum-free and protein-free processes for BHK-21 cells. Because it does not require supplementation with serum or other undefined components, Gibco CD BHK-21 Production Medium eliminates the serum associated risks while allowing you to achieve better cell growth and more consistent viral yields.
Achieve high peak cell densities:
Over four days of culture suspension BHK-21 cells achieve well over two times the densities achieved with GMEM supplemented with TPB (Tryptose Phosphate Broth) and 10% FBS.
Viral titration results in VERO cells
Viral production in triplicate plates using VERO cells grown in either Gibco VP-SFM or EMEM with 10% FBS shows equivalent results
Production of infectious bovine rhinotracheitis (IBR) virus in MDCK cells
The titer of the inoculum was 1.3 x 108 TCID50/mL. Cultures were carried for 6 subcultures in the respective medium. Cell counts shown are average counts (x 106) per flask. The length of incubation was dictated by % cytopathic effects (CPE) in serum control. The two media gave equivalent results.
Maintain high level of cell viability:
Without supplementation with FBS or TPB (Tryptose Phosphate Broth) CD BHK-21 Production Medium supports suspension BHK-21 cells longer than GMEM supported both by TPB as well as 10% FBS. Cell viabilities started to decline after 24 hours in GMEM, whereas they were stable for four days in CD BHK-21 Production Medium.
Achieve high peak cell densities:
Over four days of culture suspension BHK-21 cells achieve well over two times the densities achieved with GMEM supplemented with TPB (Tryptose Phosphate Broth) and 10% FBS.
Viral titration results in VERO cells
Viral production in triplicate plates using VERO cells grown in either Gibco VP-SFM or EMEM with 10% FBS shows equivalent results
Production of infectious bovine rhinotracheitis (IBR) virus in MDCK cells
The titer of the inoculum was 1.3 x 108 TCID50/mL. Cultures were carried for 6 subcultures in the respective medium. Cell counts shown are average counts (x 106) per flask. The length of incubation was dictated by % cytopathic effects (CPE) in serum control. The two media gave equivalent results.
Browse our catalog
The Gibco catalog also offers quality reagents, buffers and supplements for all your virus production needs.
| Animal Origin / Serum Reduced | Animal Origin Free |
Reduced-serum media
An economical solution to reduce serum is to replace it with a low serum-requiring medium, supplemented with other growth-promoting factors. Premium Gibco Opti-MEM, a Minimum Essential Medium (MEM) which is supplemented with 2%-4% FBS, actually outperforms ordinary media supplemented with 10% FBS in many critical applications. In some applications, researchers reduced serum requirements by at least 50%. Reducing serum also minimizes the risk of adventitious agents responsible for diseases, such as bovine spongiform encephalopathy (BSE).
Opti-MEM I medium outperforms EMEM with more FBS
For very low serum supplementation (<1%) with anchorage-dependent cells, Opti-MEM I medium should be supplemented with 500 to 1,000 mg/L calcium chloride. The addition of HEPES provides added pH control to the cultures.
Ordering information
Workflow production
Gibco Advanced Granulation Technology is a granular dry media format produced through a technologically advanced process which allows manufacturing of complete formulations in a variety of serum-free, protein-free and chemically-defined media in a dry format. AGT media can help reduce your total cycle costs, decreasing time involved in raw material planning, procurement and testing as well as media preparation.
Learn more about Gibco Advanced Granulation Technology
Our packaging options are flexible to meet the needs of R&D, process development, pilot plants and full-scale manufacturing, and our custom product services team can help assess feasibility and recommend design, testing and packaging options for your proprietary formulations.
Related products
Gibco BioProduction Services is available to partner with you for all of your vaccine production needs. We inspire confidence in our partnerships through our cumulative industry experience in helping customers achieve their goals with accelerated time to market. And most importantly, we believe in putting you and the needs of your customers first.
Learn more about Gibco BioProduction Services
Manufacturing a new vaccine requires absolute certainty in quality and safety. Our Applied Biosystems MycoSEQ Microbial Identification System provides the highest bacterial and fungal identification accuracy and reproducibility. Fast time-to-results helps reduce product release time. High throughput allows up to 80 identifications in less than 24 hours.
Learn more about Applied Biosystems SEQ rapid contaminant test
The development and manufacture of mission-critical biotherapeutics and vaccines demand high-performance, high-throughput bioprocessing. POROS Perfusion Chromatography medium is a unique large-pore chromatography support offering high-capacity, high-resolution bioprocessing for large molecules such as antibodies and virus-like particles.
Learn more about Chromatography and Protein Purification
Learn more about our performance vaccine production media and reagents
Learn more about Vaccine Production Media & Reagents
Step-by-step to virus expression products
We offer an integrated array of services to simplify cell line development through Gibco Bioproduction Services. From vector design to cGMP cell banking, our experienced scientists and project managers are able to provide total life cycle support.
Our chemically defined and serum-free media for virus and vaccine production deliver cell growth and virus production equivalent or superior to serum-supplemented systems while also maximizing consistency and reliability and simplifying downstream purification.
Learn more about:
POROS Chromatography resins are today’s best performing chromatographic media for process-scale bioseparations. POROS resins are available in a range of selections designed especially for today’s recombinant protein and other biological purification challenges.
Learn more about:
Applied Biosystems SEQ rapid molecular methods for pharmaceutical manufacturing help ensure the quality and safety of your pharmaceutical products especially when accuracy and time-to-results are critical. SEQ rapid molecular methods are also ideal for use in public health laboratories, cosmetics and personal care products manufacturing, food testing laboratories, and academic and research centers.
Learn more about:
Recommended products
We offer an integrated array of services to simplify cell line development through Gibco Bioproduction Services. From vector design to cGMP cell banking, our experienced scientists and project managers are able to provide total life cycle support.
Our chemically defined and serum-free media for virus and vaccine production deliver cell growth and virus production equivalent or superior to serum-supplemented systems while also maximizing consistency and reliability and simplifying downstream purification.
Learn more about:
POROS Chromatography resins are today’s best performing chromatographic media for process-scale bioseparations. POROS resins are available in a range of selections designed especially for today’s recombinant protein and other biological purification challenges.
Learn more about:
Applied Biosystems SEQ rapid molecular methods for pharmaceutical manufacturing help ensure the quality and safety of your pharmaceutical products especially when accuracy and time-to-results are critical. SEQ rapid molecular methods are also ideal for use in public health laboratories, cosmetics and personal care products manufacturing, food testing laboratories, and academic and research centers.
Learn more about:
Recommended products
Related resources
Application note: Diploid Growth Serum-Reduced Medium can reduce or eliminate serum use in human and animal vaccine production
Brochure: Streamline viral vector and vaccine development
Brochure: Vaccine Production Cell Culture
Poster: Development of a chemically defined medium for Escherichia coli, Gibco™ Bacto™ CD Supreme Fermentation Production Medium (FPM) leveraging learnings from traditional peptone-based media
Bioprocessing resources
Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing:
Support
Events & Webinars
For Research Use Only. Not for use in diagnostic procedures.