Invitrogen

HA Tag Monoclonal Antibody (2-2.2.14), DyLight™ 488

HA Tag Antibody in Immunocytochemistry (ICC/IF)

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HA Tag Antibody (26183-D488) in ICC/IF

Immunofluorescent analysis of HA Tag was performed using 70% confluent HA-H3 transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Monoclonal Antibody (2-2.2.14), DyLight 488 (Product # 26183-D488) at 1:200 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with ... View More

HA Tag Antibody in Immunohistochemistry (IHC)

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Product Details

26183-D488

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:25-1:200

View 2 publications 2 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2-2.2.14

Immunogen

HA peptide YPYDVPDYA derivitized to ovalbumin.

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533051

Product Specific Information

26183-D488 has been successfully used for immunofluorescence. DyLight 488 has an excitation/emission of 493/518 nm.

HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Conserved nuclear hormone receptors controlling a novel plastic trait target fast-evolving genes expressed in a single cell.

PLoS genetics

Sieriebriennikov B,Sun S,Lightfoot JW,Witte H,Moreno E,Rödelsperger C,Sommer RJ

26183-D488 was used in Immunohistochemistry-immunofluorescence to investigate the gene regulatory network controlling predatory vs. non-predatory dimorphism in the nematode Pristionchus pacificus.

Wed Apr 01 00:00:00 EDT 2020

Conserved nuclear hormone receptors controlling a novel plastic trait target fast-evolving genes expressed in a single cell.

PLoS genetics

Sieriebriennikov B,Sun S,Lightfoot JW,Witte H,Moreno E,Rödelsperger C,Sommer RJ

26183-D488 was used in Immunohistochemistry-immunofluorescence to investigate the gene regulatory network controlling predatory vs. non-predatory dimorphism in the nematode Pristionchus pacificus.

Wed Apr 01 00:00:00 EDT 2020

mRNA as a Novel Treatment Strategy for Hereditary Spastic Paraplegia Type 5.

Molecular therapy. Methods & clinical development

Hauser S,Poenisch M,Schelling Y,Höflinger P,Schuster S,Teegler A,Betten R,Gustafsson JÅ,Hübener-Schmid J,Schlake T,Chevessier-Tünnesen F,Horscroft N,Björkhem I,Schöls L

Published figure using HA Tag monoclonal antibody (Product # 26183-D488) in Immunocytochemistry

Fri Dec 13 00:00:00 EST 2019

Identification of VAPA and VAPB as Kv2 Channel-Interacting Proteins Defining Endoplasmic Reticulum-Plasma Membrane Junctions in Mammalian Brain Neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kirmiz M,Vierra NC,Palacio S,Trimmer JS

26183-D488 was used in Immunohistochemistry-immunofluorescence to suggests that Kv2.1 expression in the PM can affect ER-PM junctions via its phosphorylation-dependent association to ER-localized VAPA and VAPB.

Wed Aug 29 00:00:00 EDT 2018