Invitrogen

HA Tag Monoclonal Antibody (2-2.2.14), Biotin

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4 References

View all (114) HA Tag antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 6

HA Tag Antibody (26183-BTIN) in WB

Western blot was performed using Biotin-conjugated Anti-HA Tag Recombinant Mouse Monoclonal Antibody (Product # 26183-BTIN) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), HA-H3, 40 µg (Lane 3), HA-H3, 20 µg (Lane 4), HA-H3, 10 µg (Lane 5), HA-H3, 5 µg (Lane 6), p65-HA, 40 µg (Lane 7), Myc-p65-V5, 40 µg (Lane 8) and 25 ng of Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). ... View More

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Product Details

26183-BTIN

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2-2.2.14

Immunogen

HA peptide YPYDVPDYA derivitized to ovalbumin.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533050

Product Specific Information

26183-BTIN has been successfully used for Western blot and IP.

HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deletion of the Plasmodium falciparum exported protein PTP7 leads to Maurer's clefts vesiculation, host cell remodeling defects, and loss of surface presentation of EMP1.

PLoS pathogens

Carmo OMS,Shami GJ,Cox D,Liu B,Blanch AJ,Tiash S,Tilley L,Dixon MWA

26183-BTIN was used in Immunocytochemistry-immunoflourescence to describe a protein PF3D7_0301700 (PTP7), that functions at the nexus between the intermediate trafficking organelle, the Maurer's cleft, and the infected red blood cell surface.

Mon Aug 01 00:00:00 EDT 2022

^αN-Acetyl β-Endorphin Is an Endogenous Ligand of σ1Rs That Regulates Mu-Opioid Receptor Signaling by Exchanging G Proteins for σ2Rs in σ1R Oligomers.

International journal of molecular sciences

Garzón-Niño J,Cortés-Montero E,Rodríguez-Muñoz M,Sánchez-Blázquez P

26183-BTIN was used in Western blotting following immunoprecipitation to suggest that the pharmacological β-Endorphin-specific epsilon receptor is a σ1R-regulated MOR and that β-Endorphin and αN-acetyl β-Endorphin are endogenous ligands of σ1R.

Thu Dec 29 00:00:00 EST 2022

Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein.

Nature communications

Urban JH,Moosmeier MA,Aumüller T,Thein M,Bosma T,Rink R,Groth K,Zulley M,Siegers K,Tissot K,Moll GN,Prassler J

26183-BTIN was used in a study to develop tools for the systematic engineering and selection of lanthipeptides by phage display.

Wed Nov 15 00:00:00 EST 2017

Valproic acid causes proteasomal degradation of DICER and influences miRNA expression.

PloS one

Zhang Z,Convertini P,Shen M,Xu X,Lemoine F,de la Grange P,Andres DA,Stamm S

26183-BTIN was used in western blot to study the proteasomal degradation of DICER and altered miRNA levels induced by valproic acid

Tue Oct 21 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin

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