Invitrogen

CD279 (PD-1) Monoclonal Antibody (J43), Functional Grade, eBioscience™

CD279 (PD-1) Antibody in Flow Cytometry (Flow)

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CD279 (PD-1) Antibody (16-9985-82) in Flow

Staining of 3-day unstimulated (left) and 3-day ConA-stimulated (right) BALB/c splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4888-81) (open histogram) or 0.25 µg of Anti-Mouse CD279 (PD-1) Functional Grade Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD279 (PD-1) Antibody in Immunohistochemistry (IHC)

CD279 (PD-1) Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

16-9985-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 45 publications 45 publications

Neutralization (Neu)

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

J43

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469307

Product Specific Information

Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Applications Reported: The J43 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in in vitro functional assays.

Applications Tested: The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cells and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD153/CD30 signaling promotes age-dependent tertiary lymphoid tissue expansion and kidney injury.

The Journal of clinical investigation

Sato Y,Oguchi A,Fukushima Y,Masuda K,Toriu N,Taniguchi K,Yoshikawa T,Cui X,Kondo M,Hosoi T,Komidori S,Shimizu Y,Fujita H,Jiang L,Kong Y,Yamanashi T,Seita J,Yamamoto T,Toyokuni S,Hamazaki Y,Hattori M,Yoshikai Y,Boor P,Floege J,Kawamoto H,Murakawa Y,Minato N,Yanagita M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Jan 18 00:00:00 EST 2022

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy.

Journal for immunotherapy of cancer

Hu J,He J,Wang Y,Zhao Y,Fang K,Dong Y,Chen Y,Zhang Y,Zhang C,Wang H,Tan J,Wang J,Zi R,Liu C,Liang H,Guo Y,Ou J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.

Cell reports

Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.

Nature immunology

Gu M,Zhou X,Sohn JH,Zhu L,Jie Z,Yang JY,Zheng X,Xie X,Yang J,Shi Y,Brightbill HD,Kim JB,Wang J,Cheng X,Sun SC

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Thu Dec 02 00:00:00 EST 2021

Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade.

JCI insight

Ferrere G,Tidjani Alou M,Liu P,Goubet AG,Fidelle M,Kepp O,Durand S,Iebba V,Fluckiger A,Daillère R,Thelemaque C,Grajeda-Iglesias C,Alves Costa Silva C,Aprahamian F,Lefevre D,Zhao L,Ryffel B,Colomba E,Arnedos M,Drubay D,Rauber C,Raoult D,Asnicar F,Spector T,Segata N,Derosa L,Kroemer G,Zitvogel L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Mon Jan 25 00:00:00 EST 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nature communications

Sun Y,Lu D,Yin Y,Song J,Liu Y,Hao W,Qi F,Zhang G,Zhang X,Liu L,Lin Z,Liang H,Zhao X,Jin Y,Yin Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Stearoyl-CoA Desaturase-Mediated Monounsaturated Fatty Acid Availability Supports Humoral Immunity.

Cell reports

Zhou X,Zhu X,Li C,Li Y,Ye Z,Shapiro VS,Copland JA,Hitosugi T,Bernlohr DA,Sun J,Zeng H

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Jan 05 00:00:00 EST 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses.

Wellcome open research

Wu G,Murugesan G,Nagala M,McCraw A,Haslam SM,Dell A,Crocker PR

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Serum HBsAg clearance has minimal impact on CD8+ T cell responses in mouse models of HBV infection.

The Journal of experimental medicine

Fumagalli V,Di Lucia P,Venzin V,Bono EB,Jordan R,Frey CR,Delaney W,Chisari FV,Guidotti LG,Iannacone M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2020

B cells are sufficient to prime the dominant CD4+ Tfh response to Plasmodium infection.

The Journal of experimental medicine

Arroyo EN,Pepper M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Mon Feb 03 00:00:00 EST 2020

Inhibition of the Wnt/β-catenin pathway enhances antitumor immunity in ovarian cancer.

Therapeutic advances in medical oncology

Doo DW,Meza-Perez S,Londoño AI,Goldsberry WN,Katre AA,Boone JD,Moore DJ,Hudson CT,Betella I,McCaw TR,Gangrade A,Bao R,Luke JJ,Yang ES,Birrer MJ,Starenki D,Cooper SJ,Buchsbaum DJ,Norian LA,Randall TD,Arend RC

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2022

Gene silencing of indoleamine 2,3-dioxygenase 1 inhibits lung cancer growth by suppressing T-cell exhaustion.

Oncology letters

Shang K,Wang Z,Hu Y,Huang Y,Yuan K,Yu Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease.

Cell reports

Nguyen HD,Ticer T,Bastian D,Kuril S,Li H,Du H,Yan C,Yu XZ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Oct 27 00:00:00 EDT 2020

5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma.

Frontiers in immunology

Ebelt ND,Zuniga E,Johnson BL,Diamond DJ,Manuel ER

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses.

Antioxidants (Basel, Switzerland)

Lee HJ,Park JS,Yoo HJ,Lee HM,Lee BC,Kim JH

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2020

Endogenous retroviral proteins provide an immunodominant but not requisite antigen in a murine immunotherapy tumor model.

Oncoimmunology

Ye X,Waite JC,Dhanik A,Gupta N,Zhong M,Adler C,Malahias E,Ni M,Wei Y,Gurer C,Zhang W,Macdonald LE,Murphy AJ,Sleeman MA,Skokos D

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Wed May 13 00:00:00 EDT 2020

Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma.

Cell reports

Burrack AL,Spartz EJ,Raynor JF,Wang I,Olson M,Stromnes IM

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Aug 20 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

PD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine.

Journal of Cancer

Zhang X,Shi X,Li J,Mo L,Hu Z,Gao J,Wu S,Long Z

16-9985 was used in Flow cytometry/Cell sorting to investigate whether PD-L1 mediated adaptive resistance could occur in treatment with Anchored-GM-CSF vaccine and whether PD-1 blockade combined with Anchored-GM-CSF vaccine could induce a greater anti-tumour immune response than either immunotherapy alone.

Thu Oct 01 00:00:00 EDT 2020

PD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine.

Journal of Cancer

Zhang X,Shi X,Li J,Mo L,Hu Z,Gao J,Wu S,Long Z

Thu Oct 01 00:00:00 EDT 2020

Homeostatic control of metabolic and functional fitness of T_reg cells by LKB1 signalling.

Nature

Yang K,Blanco DB,Neale G,Vogel P,Avila J,Clish CB,Wu C,Shrestha S,Rankin S,Long L,Kc A,Chi H

16-9985-82 was used in Functional assay to show that liver kinase B1 programs the metabolic and functional fitness of Treg cells in the control of immune tolerance and homeostasis.

Thu Aug 31 00:00:00 EDT 2017

Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination.

Cancer research

Jiang H,Rivera-Molina Y,Gomez-Manzano C,Clise-Dwyer K,Bover L,Vence LM,Yuan Y,Lang FF,Toniatti C,Hossain MB,Fueyo J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Sat Jul 15 00:00:00 EDT 2017

Cblb-deficient T cells are less susceptible to PD-L1-mediated inhibition.

Oncotarget

Peer S,Baier G,Gruber T

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Jun 27 00:00:00 EDT 2017

Therapeutic efficacy of combined vaccination against tumor pericyte-associated antigens DLK1 and DLK2 in mice.

Oncoimmunology

Fabian KP,Chi-Sabins N,Taylor JL,Fecek R,Weinstein A,Storkus WJ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1⁺T_reg cells.

Nature communications

Liu Y,Marin A,Ejlerskov P,Rasmussen LM,Prinz M,Issazadeh-Navikas S

16-9985 was used in Neutralization experiments to investigate the role of IFN in generation of FoxA1(+)Treg cells.

Mon Apr 24 00:00:00 EDT 2017

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression.

Oncoimmunology

Chen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.

Oncoimmunology

Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The E-Id protein axis modulates the activities of the PI3K-AKT-mTORC1-Hif1a and c-myc/p19Arf pathways to suppress innate variant TFH cell development, thymocyte expansion, and lymphomagenesis.

Genes & development

Miyazaki M,Miyazaki K,Chen S,Chandra V,Wagatsuma K,Agata Y,Rodewald HR,Saito R,Chang AN,Varki N,Kawamoto H,Murre C

16-9985 was used in Flow cytometry/Cell sorting to show that Id2 and Id3 suppress the development and expansion of innate variant follicular helper T cells.

Sun Feb 15 00:00:00 EST 2015

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2016

Glial cells suppress postencephalitic CD8+ T lymphocytes through PD-L1.

Glia

Schachtele SJ,Hu S,Sheng WS,Mutnal MB,Lokensgard JR

16-9985 was used in Functional assays to demonstrate that microglia and astrocytes control antiviral T-cell responses.

Wed Oct 01 00:00:00 EDT 2014

The DC-HIL/syndecan-4 pathway regulates autoimmune responses through myeloid-derived suppressor cells.

Journal of immunology (Baltimore, Md. : 1950)

Chung JS,Tamura K,Akiyoshi H,Cruz PD,Ariizumi K

16-9985 was used in Flow cytometry/Cell sorting to investigate the mechanisms of regulation of autoimmune responses in experimental autoimmune encephalomyelitis.

Sat Mar 15 00:00:00 EDT 2014

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Tue Oct 28 00:00:00 EDT 2014

Blockade of the negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis.

Critical care (London, England)

Chang KC,Burnham CA,Compton SM,Rasche DP,Mazuski RJ,McDonough JS,Unsinger J,Korman AJ,Green JM,Hotchkiss RS

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Sat May 11 00:00:00 EDT 2013

Programmed death receptor-1/programmed death receptor ligand-1 blockade after transient lymphodepletion to treat myeloma.

Journal of immunology (Baltimore, Md. : 1950)

Kearl TJ,Jing W,Gershan JA,Johnson BD

16-9985 was used in Flow cytometry/Cell sorting to demonstrate that a blockade of the PD-1/PD-L1 pathway yields positive antitumour results.

Sat Jun 01 00:00:00 EDT 2013

ICAM-1-dependent homotypic aggregates regulate CD8 T cell effector function and differentiation during T cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Zumwalde NA,Domae E,Mescher MF,Shimizu Y

16-9985 was used in Flow cytometry/Cell sorting to demonstrate that during CD8 T cell activation, the T cell clustering mechanism allows for continued proliferation of T cells, and regulates CD8 T cell effector function and differentiation.

Tue Oct 01 00:00:00 EDT 2013

Sequential induction of effector function, tissue migration and cell death during polyclonal activation of mouse regulatory T-cells.

PloS one

Langenhorst D,Gogishvili T,Ribechini E,Kneitz S,McPherson K,Lutz MB,Hünig T

16-9985 was used in Flow cytometry/Cell sorting to investigate the origin and fate of IL-10 producing Tregs.

Mon May 20 00:00:00 EDT 2013

Farnesyltransferase inhibitor FTI-277 reduces mortality of septic mice along with improved bacterial clearance.

The Journal of pharmacology and experimental therapeutics

Yang W,Yamada M,Tamura Y,Chang K,Mao J,Zou L,Feng Y,Kida K,Scherrer-Crosbie M,Chao W,Ichinose F,Yu YM,Fischman AJ,Tompkins RG,Yao S,Kaneki M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Regulation of T cell priming by lymphoid stroma.

PloS one

Khan O,Headley M,Gerard A,Wei W,Liu L,Krummel MF

16-9985 was used in Flow cytometry/Cell sorting to show that murine fibroblastic reticular cells (FRCs) play a more direct role in the immune response by sensing and modulating T cell activation.

Mon Mar 26 00:00:00 EDT 2012

B and T lymphocyte attenuator suppresses IL-21 production from follicular Th cells and subsequent humoral immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Kashiwakuma D,Suto A,Hiramatsu Y,Ikeda K,Takatori H,Suzuki K,Kagami S,Hirose K,Watanabe N,Iwamoto I,Nakajima H

16-9985 was used in Flow cytometry/Cell sorting to suggest that B and T lymphocyte attenuator signaling suppresses IL-21 production by Tfh cells and subsequent Tfh cell-mediated IgG responses.

Wed Sep 01 00:00:00 EDT 2010

Murine isolated lymphoid follicles contain follicular B lymphocytes with a mucosal phenotype.

American journal of physiology. Gastrointestinal and liver physiology

Wang C,McDonald KG,McDonough JS,Newberry RD

16-9985 was used in Flow cytometry/Cell sorting to indicate that murine ILFs contain a polyclonal population of follicular B-2 B lymphocytes with a phenotype similar to PP B lymphocytes.

Sun Oct 01 00:00:00 EDT 2006

Local expression of B7-H1 promotes organ-specific autoimmunity and transplant rejection.

The Journal of clinical investigation

Subudhi SK,Zhou P,Yerian LM,Chin RK,Lo JC,Anders RA,Sun Y,Chen L,Wang Y,Alegre ML,Fu YX

16-9985 was used in Flow cytometry/Cell sorting to determine whether local nonlymphoid tissue expression of B7-H1 inhibits T cell responses and autoimmunity in vivo using transgenic mice.

Mon Mar 01 00:00:00 EST 2004

Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis.

The Journal of experimental medicine

Salama AD,Chitnis T,Imitola J,Ansari MJ,Akiba H,Tushima F,Azuma M,Yagita H,Sayegh MH,Khoury SJ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 16-9985-82) in Immunohistochemistry

Mon Jul 07 00:00:00 EDT 2003

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 EDT 2003

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 EDT 2003

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 EDT 2003

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: CD279; hPD1; mPD-1; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279

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Gene Aliases: Ly101; PD-1; Pd1; Pdc1; Pdcd1

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UniProt ID: (Mouse) Q02242

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Entrez Gene ID: (Mouse) 18566

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Function(s)

protein binding

Process(es)

immune system process negative regulation of tolerance induction apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of T cell apoptotic process

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CD279 (PD-1) Monoclonal Antibody (J43), Functional Grade, eBioscience™

CD279 (PD-1) Antibody (16-9985-82) in Flow

Product Details

16-9985-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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