Invitrogen

CD279 (PD-1) Monoclonal Antibody (J43), APC, eBioscience™

CD279 (PD-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 45

CD279 (PD-1) Antibody (17-9985-82) in Flow

Staining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (blue histogram) or 0.25 µg of Anti-Mouse CD279 (PD-1) APC (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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Product Details

17-9985-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 43 publications 43 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

J43

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149358

Product Specific Information

Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Applications Reported: This J43 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This J43 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Single-cell RNA sequencing reveals the suppressive effect of PPP1R15A inhibitor Sephin1 in antitumor immunity.

iScience

Wang R,Zhang Y,Guo S,Pei S,Guo W,Wu Z,Wang H,Wang M,Li Y,Zhu Y,Meng LH,Lang J,Jin G,Xiao Y,Hu L,Kong X

17-9985-82 was used in Flow cytometry/Cell sorting to demonstrate that an inhibitor of PPP1R15A, Sephin1, plays a protumorigenic role in mouse tumor models.

Fri Feb 17 00:00:00 EST 2023

Microbiota-assisted iron uptake promotes immune tolerance in the intestine.

Nature communications

Zhu L,Li G,Liang Z,Qi T,Deng K,Yu J,Peng Y,Zheng J,Song Y,Chang X

17-9985-82 was used in Flow cytometry/Cell sorting to reveal an association between nutrient uptake and immune tolerance in the intestine.

Mon May 15 00:00:00 EDT 2023

CD153/CD30 signaling promotes age-dependent tertiary lymphoid tissue expansion and kidney injury.

The Journal of clinical investigation

Sato Y,Oguchi A,Fukushima Y,Masuda K,Toriu N,Taniguchi K,Yoshikawa T,Cui X,Kondo M,Hosoi T,Komidori S,Shimizu Y,Fujita H,Jiang L,Kong Y,Yamanashi T,Seita J,Yamamoto T,Toyokuni S,Hamazaki Y,Hattori M,Yoshikai Y,Boor P,Floege J,Kawamoto H,Murakawa Y,Minato N,Yanagita M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Jan 18 00:00:00 EST 2022

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy.

Journal for immunotherapy of cancer

Hu J,He J,Wang Y,Zhao Y,Fang K,Dong Y,Chen Y,Zhang Y,Zhang C,Wang H,Tan J,Wang J,Zi R,Liu C,Liang H,Guo Y,Ou J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.

Cell reports

Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141⁺ cancer-associated fibroblasts in pancreatic cancer.

Nature communications

Kim DK,Jeong J,Lee DS,Hyeon DY,Park GW,Jeon S,Lee KB,Jang JY,Hwang D,Kim HM,Jung K

17-9985-82 was used in Flow cytometry/Cell sorting to study the mechanism underlying chemotherapy-induced fibrosis in PDAC.

Sat Oct 22 00:00:00 EDT 2022

Expansion of T memory stem cells with superior anti-tumor immunity by Urolithin A-induced mitophagy.

Immunity

Denk D,Petrocelli V,Conche C,Drachsler M,Ziegler PK,Braun A,Kress A,Nicolas AM,Mohs K,Becker C,Neurath MF,Farin HF,Buchholz CJ,Andreux PA,Rinsch C,Greten FR

17-9985-82 was used in Sample Preparation to unravel a critical signaling pathway linking mitophagy to T memory stem cell formation and suggest that the metabolic compound Urolithin A represents an option to improve immune therapy.

Tue Nov 08 00:00:00 EST 2022

NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.

Nature immunology

Gu M,Zhou X,Sohn JH,Zhu L,Jie Z,Yang JY,Zheng X,Xie X,Yang J,Shi Y,Brightbill HD,Kim JB,Wang J,Cheng X,Sun SC

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

17-9985-82 was used in Flow Cytometry to elucidate the molecular mechanisms regulating T follicular helper cells.

Thu Apr 01 00:00:00 EDT 2021

Ileal immune tonus is a prognosis marker of proximal colon cancer in mice and patients.

Cell death and differentiation

Picard M,Yonekura S,Slowicka K,Petta I,Rauber C,Routy B,Richard C,Iebba V,Tidjani Alou M,Becharef S,Ly P,Pizzato E,Lehmann CHK,Amon L,Klein C,Opolon P,Gomperts Boneca I,Scoazec JY,Hollebecque A,Malka D,Ghiringhelli F,Dudziak D,Berx G,Vereecke L,van Loo G,Kroemer G,Zitvogel L,Roberti MP

17-9985 was used in Flow cytometry/Cell sorting to suggest that chemotherapy inversely shapes the immune profile of the ileum-tumor axis, influencing clinical outcome.

Sat May 01 00:00:00 EDT 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Thu Dec 02 00:00:00 EST 2021

Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade.

JCI insight

Ferrere G,Tidjani Alou M,Liu P,Goubet AG,Fidelle M,Kepp O,Durand S,Iebba V,Fluckiger A,Daillère R,Thelemaque C,Grajeda-Iglesias C,Alves Costa Silva C,Aprahamian F,Lefevre D,Zhao L,Ryffel B,Colomba E,Arnedos M,Drubay D,Rauber C,Raoult D,Asnicar F,Spector T,Segata N,Derosa L,Kroemer G,Zitvogel L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Jan 25 00:00:00 EST 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nature communications

Sun Y,Lu D,Yin Y,Song J,Liu Y,Hao W,Qi F,Zhang G,Zhang X,Liu L,Lin Z,Liang H,Zhao X,Jin Y,Yin Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Stearoyl-CoA Desaturase-Mediated Monounsaturated Fatty Acid Availability Supports Humoral Immunity.

Cell reports

Zhou X,Zhu X,Li C,Li Y,Ye Z,Shapiro VS,Copland JA,Hitosugi T,Bernlohr DA,Sun J,Zeng H

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Jan 05 00:00:00 EST 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses.

Wellcome open research

Wu G,Murugesan G,Nagala M,McCraw A,Haslam SM,Dell A,Crocker PR

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Serum HBsAg clearance has minimal impact on CD8+ T cell responses in mouse models of HBV infection.

The Journal of experimental medicine

Fumagalli V,Di Lucia P,Venzin V,Bono EB,Jordan R,Frey CR,Delaney W,Chisari FV,Guidotti LG,Iannacone M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2020

Serum HBsAg clearance has minimal impact on CD8+ T cell responses in mouse models of HBV infection.

The Journal of experimental medicine

Fumagalli V,Di Lucia P,Venzin V,Bono EB,Jordan R,Frey CR,Delaney W,Chisari FV,Guidotti LG,Iannacone M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2020

B cells are sufficient to prime the dominant CD4+ Tfh response to Plasmodium infection.

The Journal of experimental medicine

Arroyo EN,Pepper M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Feb 03 00:00:00 EST 2020

Inhibition of the Wnt/β-catenin pathway enhances antitumor immunity in ovarian cancer.

Therapeutic advances in medical oncology

Doo DW,Meza-Perez S,Londoño AI,Goldsberry WN,Katre AA,Boone JD,Moore DJ,Hudson CT,Betella I,McCaw TR,Gangrade A,Bao R,Luke JJ,Yang ES,Birrer MJ,Starenki D,Cooper SJ,Buchsbaum DJ,Norian LA,Randall TD,Arend RC

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2022

Gene silencing of indoleamine 2,3-dioxygenase 1 inhibits lung cancer growth by suppressing T-cell exhaustion.

Oncology letters

Shang K,Wang Z,Hu Y,Huang Y,Yuan K,Yu Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Gene silencing of indoleamine 2,3-dioxygenase 1 inhibits lung cancer growth by suppressing T-cell exhaustion.

Oncology letters

Shang K,Wang Z,Hu Y,Huang Y,Yuan K,Yu Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease.

Cell reports

Nguyen HD,Ticer T,Bastian D,Kuril S,Li H,Du H,Yan C,Yu XZ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Oct 27 00:00:00 EDT 2020

5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma.

Frontiers in immunology

Ebelt ND,Zuniga E,Johnson BL,Diamond DJ,Manuel ER

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses.

Antioxidants (Basel, Switzerland)

Lee HJ,Park JS,Yoo HJ,Lee HM,Lee BC,Kim JH

17-9985-82 was used in Flow Cytometry to show methionine sulfoxide reductase B1 (MsrB1) acts in dendritic cells to regulate the phosphorylation of signal transducer and activator of transcription-6 (STAT6).

Tue Oct 20 00:00:00 EDT 2020

The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses.

Antioxidants (Basel, Switzerland)

Lee HJ,Park JS,Yoo HJ,Lee HM,Lee BC,Kim JH

Tue Oct 20 00:00:00 EDT 2020

Endogenous retroviral proteins provide an immunodominant but not requisite antigen in a murine immunotherapy tumor model.

Oncoimmunology

Ye X,Waite JC,Dhanik A,Gupta N,Zhong M,Adler C,Malahias E,Ni M,Wei Y,Gurer C,Zhang W,Macdonald LE,Murphy AJ,Sleeman MA,Skokos D

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Wed May 13 00:00:00 EDT 2020

Dynamics and genomic landscape of CD8⁺ T cells undergoing hepatic priming.

Nature

Bénéchet AP,De Simone G,Di Lucia P,Cilenti F,Barbiera G,Le Bert N,Fumagalli V,Lusito E,Moalli F,Bianchessi V,Andreata F,Zordan P,Bono E,Giustini L,Bonilla WV,Bleriot C,Kunasegaran K,Gonzalez-Aseguinolaza G,Pinschewer DD,Kennedy PTF,Naldini L,Kuka M,Ginhoux F,Cantore A,Bertoletti A,Ostuni R,Guidotti LG,Iannacone M

17-9985 was used in Flow cytometry/Cell sorting to show that priming by Kupffer cells, which are not natural targets of hepatitis B, leads to differentiation of CD8+ T cells into effector cells that form dense, extravascular clusters of immotile cells scattered throughout the liver.

Tue Oct 01 00:00:00 EDT 2019

Orphan Nuclear Receptor NR2F6 Suppresses T Follicular Helper Cell Accumulation through Regulation of IL-21.

Cell reports

Olson WJ,Jakic B,Labi V,Schoeler K,Kind M,Klepsch V,Baier G,Hermann-Kleiter N

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Sep 10 00:00:00 EDT 2019

Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma.

Cell reports

Burrack AL,Spartz EJ,Raynor JF,Wang I,Olson M,Stromnes IM

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Aug 20 00:00:00 EDT 2019

CXCR5⁺PD-1⁺ follicular helper CD8 T cells control B cell tolerance.

Nature communications

Chen Y,Yu M,Zheng Y,Fu G,Xin G,Zhu W,Luo L,Burns R,Li QZ,Dent AL,Zhu N,Cui W,Malherbe L,Wen R,Wang D

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Fri Sep 27 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

PD-1 Blockade and CD27 Stimulation Activate Distinct Transcriptional Programs That Synergize for CD8⁺ T-Cell-Driven Antitumor Immunity.

Clinical cancer research : an official journal of the American Association for Cancer Research

Buchan SL,Fallatah M,Thirdborough SM,Taraban VY,Rogel A,Thomas LJ,Penfold CA,He LZ,Curran MA,Keler T,Al-Shamkhani A

17-9985 was used in Flow cytometry/Cell sorting to show that suboptimal T-cell invigoration in cancer patients undergoing treatment with PD-1 checkpoint blockers will be improved by dual PD-1 blockade and CD27 agonism.

Tue May 15 00:00:00 EDT 2018

Orally administered heat-killed Lactobacillus paracasei MCC1849 enhances antigen-specific IgA secretion and induces follicular helper T cells in mice.

PloS one

Arai S,Iwabuchi N,Takahashi S,Xiao JZ,Abe F,Hachimura S

17-9985 was used in Flow cytometry/Cell sorting to investigate the effects of orally administered heat-killed MCC1849 on IgA production in the intestine and on Tfh cell induction in vivo.

Wed Jan 02 00:00:00 EST 2019

Intravenous Labeling and Analysis of the Content of Thymic Perivascular Spaces.

Bio-protocol

Ruscher R,Hogquist KA

17-9985 was used in Flow cytometry/Cell sorting to investigate hematopoietic cell subsets within the perivascular spaces of the thymus.

Mon Mar 05 00:00:00 EST 2018

Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination.

Cancer research

Jiang H,Rivera-Molina Y,Gomez-Manzano C,Clise-Dwyer K,Bover L,Vence LM,Yuan Y,Lang FF,Toniatti C,Hossain MB,Fueyo J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Sat Jul 15 00:00:00 EDT 2017

Cblb-deficient T cells are less susceptible to PD-L1-mediated inhibition.

Oncotarget

Peer S,Baier G,Gruber T

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Jun 27 00:00:00 EDT 2017

Therapeutic efficacy of combined vaccination against tumor pericyte-associated antigens DLK1 and DLK2 in mice.

Oncoimmunology

Fabian KP,Chi-Sabins N,Taylor JL,Fecek R,Weinstein A,Storkus WJ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

17-9985 was used in Flow cytometry/Cell sorting to support that Stat3- and BCL6-dependent extrafollicular CD4+ T and B interactions play critical functions in the pathogenesis of chronic graft-versus-host disease.

Tue Oct 17 00:00:00 EDT 2017

Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression.

Oncoimmunology

Chen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.

Oncoimmunology

Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9985-82) in Flow Cytometry

Tue Oct 28 00:00:00 EDT 2014

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 EDT 2003

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 EDT 2003

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: CD279; hPD1; mPD-1; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279

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Gene Aliases: Ly101; PD-1; Pd1; Pdc1; Pdcd1

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UniProt ID: (Mouse) Q02242

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Entrez Gene ID: (Mouse) 18566

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Function(s)

protein binding

Process(es)

immune system process negative regulation of tolerance induction apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of T cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD279 (PD-1) Monoclonal Antibody (J43), APC, eBioscience™

CD279 (PD-1) Antibody (17-9985-82) in Flow

Product Details

17-9985-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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