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Invitrogen
Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™
Product Details
17-4888-82
Flow Cytometry (Flow)
Control (Ctrl)
Host/Isotype
Class
Type
Clone
Conjugate
Excitation/Emission Max
Form
Concentration
Purification
Storage buffer
Contains
Storage conditions
Shipping conditions
RRID
Product Specific Information
Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis. Isotype control should be used at the same concentration as experimental antibody.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG; Immunoglobulin G; ImmunoglobulinG
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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