Rat ovarian granulosa cells.

Selective uptake of cholesteryl esters (CE) in rat ovarian granulosa cells as monitored with cholesteryl BODIPY® FL C12 (Cat. No. C3927MP). The hormone-stimulated cells internalized and stored CEs derived from reconstituted high-density lipoprotein (HDL)–BODIPY® CE complexes (J Biol Chem 271, 16208 (1996)). A low-light (<100 µW beam power) computerized imaging system minimized any photobleaching of the fluorophore. This pseudocolored image uses yellow-green to illustrate the low-level fluorescence of the cytoplasmic membranes, yellow to illustrate the medium-level fluorescence of the Golgi, and red to illustrate the high-level fluorescence of the lipid droplets. Image contributed by Eve Reaven, VA Medical Center, Palo Alto, California.

Selective uptake of cholesteryl esters (CE) in rat ovarian granulosa cells as monitored with cholesteryl BODIPY® FL C<sub>12</sub> (Cat. No. C3927MP). The hormone-stimulated cells internalized and stored CEs derived from reconstituted high-density lipoprotein (HDL)–BODIPY® CE complexes (J Biol Chem 271, 16208 (1996)). A low-light (<100 µW beam power) computerized imaging system minimized any photobleaching of the fluorophore. This pseudocolored image uses yellow-green to illustrate the low-level fluorescence of the cytoplasmic membranes, yellow to illustrate the medium-level fluorescence of the Golgi, and red to illustrate the high-level fluorescence of the lipid droplets. Image contributed by Eve Reaven, VA Medical Center, Palo Alto, California.

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