YM Medium, powder
INTENDED USE: Growth and expression of Agrobacterium. YM Medium improves transformation efficiency for electroporation of Agrobacterium by up to 40-fold when compared with LB broth. Also, no aggregation of Agrobacterium was observed when YM Medium was used in culturing the bacteria for plant infection. INSTRUCTIONS FOR USE: Suspend contents of 1 packet in 1 L of demineralized water. Autoclave for 15 min at 121°C. To prepare YM agar: Suspend contents of 1 packet in 1 L of demineralized water. Add 15 g of SELECT Agar and boil while stirring to dissolve all ingredients completely. Autoclave for 15 min at 121°C. Cool down to 50C before adding antibiotics (optional) and pouring into plates. RECOMMENDED STORAGE CONDITION: 15°C TO 30°C.
Catalog Number(s)
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Inorganic Salts | |||
| Dipotassium hydrogen phosphate | 0.5 | Infinity | |
| Magnesium Sulfate (MgSO4-7H2O) | 0.2 | Infinity | |
| Sodium Chloride (NaCl) | 0.1 | Infinity | |
| Other Components | |||
| Mannitol | 10.0 | Infinity | |
| SELECT Yeast Extract | 0.4 | Infinity | |
Reference:
1. Lin J.J. (1994) Focus® 16, 18.
2. Lin J.J. (1994) Plant Science 10, 11.
3. Lin J.J. Assad-Garcia, N. and Kuo, J. (1994) Focus® 16, 72.
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