Theory and basics
This introduction covers the theory and techniques of carbohydrate separations using HPAE-PAD. Covering the history, importance and chemistry of these challenging separations.
Getting started
Whether you are new to ion chromatography for carbohydrate analysis and looking for a guide to get you started, or an experienced user wanting to take your analysis to the next level, you will find the information here to help you make the right choices.
Take one of our on-line learning modules to understand the fundamentals of carbohydrate analysis using high performance anion exchange with pulsed amperometric detection (HPAE-PAD) covering the theory and basics of the technique, why eluent preparation is so important and which column is the right choice for your application.
Online learning
Learn more about the basics of carbohydrate analysis using high performance anion exchange with pulsed amporemetric detection (HPAE-PAD) in these on-line tutorials.
Eluent preparation
This module covers the importance of preparing eluents for carbohydrate analysis correctly. Eluents for carbohydrates are particularly challenging due to the affinity of bacteria for high sugar solutions.
Select the right column
This webinar covers how to select the correct column for your carbohydrate type. Choosing the correct column capacity for your matrix is essential for proper carbohydrate analysis.
Column technology
Determine the right column for you
This handy columns selection guide for carbohydrates analysis will help you determine which is the right column for you.
Learn more about carbohydrates in food testing
If you are involved in food testing, head to our dedicated carbohydrates testing in food page for more detailed information on food applications.
Glycan analysis
Complex carbohydrate analysis
If you are looking at more complex carbohydrates such as oligosaccharides or glycans, learn how the latest column technology can help: Filling the gaps in glycan analysis with the Thermo ScientificTM DionexTM CarboPacTM PA300 column.
Here we will find out about the Dionex CarboPac PA300 column and what areas we can resolve to understand glycans better, whether it's O-linked or N-linked, and if it's charged or neutral.
Advancing eluents
Using eluent generation to advance carbohydrate analysis
Simplify the complexity of carbohydrate analysis with eluent generation
From simple mono- and disaccharides to the more complex oligosaccharides - you often require multiple method conditions to separate all compounds.
This means a range of eluents and buffers may be required. Sourcing the purest chemicals to use for eluents can be challenging. Many cautious steps are needed to manually prepare eluents to ensure good reproducible data. Method development can take longer due to multiple eluent make ups.
Automated eluent generation can simplify carbohydrate analysis by providing:
- Cleaner and purer eluents
- Reproducible eluents, day to day, user to user, lab to lab
- Minimal need for maintenance to your instrument
- Time and resource savings
- Smarter working and increased productivity
- Ability to run gradients on an isocratic pump.
What is eluent generation?
What exactly does eluent generation mean?
This whitepaper explains how eluent generation is an electrolytic process allowing the production of high purity eluents for ion chromatography, also known as Reagent Free ion chromatography. The entire process only requires deionized water, eliminating the need to handle any acids or bases.
How does eluent generation work? This whiteboard video shows the chemistry behind eluent generation, the types of eluents that can be generated and the benefits this can bring to your workflows. Understand the role eluent generation plays in ion chromatography.
What is dual eluent generation?
Dual eluent generation can be used for separations where sodium acetate buffers are usually required.
As well as the benefits of eluent generation detailed above, dual eluent generation also enables you to:
- 'Just add water' to analyse complex carbohydrates without the need for sodium hydroxide/ sodium acetate gradients
- Eliminate the risk of contamination from lower quality sodium acetate.
Find out more about dual eluent generation
Blog post: Is two sweeter than one?
This blog post discusses the benefits of eluent generation for carbohydrate analysis as well as the latest advances with dual eluent generation, which can enable you to profile more carbohydrates from mono to oligosaccharides in one run.
Could dual eluent generation save you money?
The Dual EGC Mode Cost Savings Calculator helps you estimate your yearly savings when running your Thermo Scientific Dionex ICS-6000 HPIC system in Dual EGC mode rather than preparing eluents manually.
Further information on complex carbohydrate analysis
Related application notes
A glycopeptide standard for improving glycoprotein hydrolysis reaction yield for accurate monosaccharide content determination
See the application of a glycopeptide standard for improving accuracy of glycoprotein monosaccharide quantification using HPAE-PAD on a Thermo Scientific Dionex CarboPac PA20-4µm-Fast column.
HPAE-PAD analysis of N-linked glycans: improving glycan resolution
The goal of this application is to demonstrate conditions for improved resolution of sialylated N-glycans on Thermo Scientific Dionex CarboPac PA200 columns.
HPAE-PAD profiling of N-linked oligosaccharides from glycoproteins using dual-eluent generation cartridges
See the Dual EGC capability and performance for profiling N-linked oligosaccharides released from glycoproteins using high-performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD).
Blog post
Reproducible analysis of N-linked glycans using dual eluent generation cartridge mode HPAE-PAD.
Hear how our customers at SGS Life Sciences, West Chester, USA are using ion chromatography to profile glycans in biological products.
Looking to advance even further with HPAE-PAD and mass spectrometry?
Webinar: Towards a more complete glycome - Advances in ion chromatography-mass spectrometry (IC-MS) for improved separation and analysis of carbohydrates
This on-demand webinar details how HPAE-PAD can be linked to mass spectrometry to advance carbohydrate analysis to the maximum to access data on the acidic subsets of the glycome to compliment LC-MS based methods.
Learn more about glycan and biopharmaceutical applications ›
Tips & Tricks for carbohydrate analysis
Are you an existing user and need some practical support, find out more here
Troubleshooting your carbohydrate analysis
In this section we will cover best practices for maintaining your system for optimized output, and how to troubleshoot common carbohydrate analysis problems, such as poor signal-to-noise ratios, pressure increases and poor peak retention.
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