Small amounts of RNases can sometimes co-purify with isolated RNA and compromise downstream applications. These contaminants can be introduced via tips, tubes, and other reagents. RNase inhibitors are used to inhibit and control contaminants.
Once RNA is quantified, you are ready for reverse transcription. The ideal reverse transcriptase (RT) will reverse-transcribe even the most difficult RNAs, such as those degraded during the purification process or those in limited quantity. Sensitivity, consistency, thermostability, and speed are key attributes to consider when selecting the best RT for your experiment. This is a critical step in gene expression analysis and Thermo Fisher Scientific can be relied upon to deliver products that meet every research need and to produce accurate gene expression results.
Oligonucleotides (oligos) and probes are used at many points within gene expression workflows. While the technology is well established, degraded, or nonspecific oligos can ruin your results. Choose from a wide variety of pre-designed and custom primers and probes, available with more than 50 modifications including MGB, TAMRA, and QSY.
Choose from a variety of reverse transcription enzymes and formats, for superior cDNA synthesis results with any type of template RNA. Enjoy proven results, even with the presence of inhibitors or partially degraded RNA, allowing you to salvage suboptimal samples.
Which SuperScript product is right for you?
Most effective enzyme for all RNA, including difficult templates | Full optimization with flexible priming options for RT-PCR | Highest performance and convenience for RT-qPCR | Highest performance and convenience for RT-qPCR; with integrated gDNA removal | Fast and simple RT-PCR workflow | |
| Product | |||||
Format | Stand-alone enzyme | cDNA synthesis kit | cDNA synthesis master mix | cDNA synthesis master mix with gDNA removal | One-step RT-PCR kit |
Optimal reaction temperature | 50–55°C | 50–55°C | 50°C | 50°C | 50-55°C |
RT reaction time | 10 min | 10 min | 10 min | 10 min (additional 2 min gDNA removal) | 10 min |
Protocol |
Gene Expression | RNA extraction | RNA Quantification | Reverse Transcription | PCR | QuantiGene |
Style Sheet for Global Design System
Style Sheet for Komodo Tabs