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Use this online tool to transfer methods from HPLC to UHPLC conditions. The tool will calculate the new gradient table, adjust injection volumes, flow rates and run times if applicable. Please insert your current method conditions and find out how much time, sample and solvents you could save using UHPLC.

Use at your own risk. Prepopulated data are estimates only. Thermo Fisher Scientific takes no responsibility for the accuracy of data, calculations or results.

Current Method

Current Column

Length (mm):

Diameter (mm):

Particle Size (µm):

UHPLC Method

Planned Column

Length (mm):

Diameter (mm):

Particle Size (µm):

Peak Details (Critical Pair)

Actual R_s: (Resolution Factor)

Peak Details (Critical Pair)

Predicted R_s Change Factor:

Predicted R_s:

Current Method Conditions

Flow (mL/min):

Injection Volume (µL):

Max Observed Pressure:

Pressure Units:

Number of Samples:

Data Collection Rate (Hz):

Gradient Delay Vol:

Gradient Delay Volume (µL):

Recommended Method Conditions

* Boost Factor:

* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.

Flow (mL/min):

Injection Volume (µL):

Estimated Max Observed Pressure:

0 bar

Number of Samples:

Data Collection Rate (Hz):

0 Hz

Gradient Delay Volume (µL):

Optimal GDV (µL):

Adjust Flow:

Current Gradient Table

Step	Time (min)	%A
1		100.0
2
3
4
5
6
7
8
9
10
End Time:	0.000
Recommended Reconditioning Time: n.a.

Planned Gradient Table

Time (min)	%A	%B	%C	%D
0.00	100.0









End Time:	0.000
Recommended Reconditioning Time: n.a.

Sample usage savings

Current Usage	Planned Usage	Savings

Eluent usage and time savings

	Current Total	Planned Total	Savings	Throughput
Eluent Usage
Time

HPLC Method Transfer Calculator

Current Method

UHPLC Method

Current Gradient Table

Planned Gradient Table

Sample usage savings

Eluent usage and time savings

Total Time Savings

Total Eluent Savings

Total Sample Usage Savings