Dr. Jennifer Empey, Applications Scientist, UV-Vis Spectroscopy, Thermo Fisher Scientific
Jennifer Empey has been with Thermo Fisher Scientific as an Applications Scientist since October, 2021. She supports the Core UV-Visible instruments. Prior to this position, she received her PhD in Analytical Chemistry from The Ohio State University, where she used various spectroscopic techniques to study colloidal nanoparticles.
Webinar
DNA melting temperature analysis
Double-stranded DNA can play an important role in a variety of pharmaceutical formulation procedures, including vaccine development. The melting temperature, or temperature at which the double-stranded DNA disassociates into its single-stranded components, is characteristic of the specific DNA sequence used and can be determined through temperature-dependent UV-visible absorption measurements.
The observed melting temperature of a DNA sequence can be sensitive to changes in the buffer concentration, the species present in solution, and the presence of base-pair mismatches within the sequence. As a result, the determination of a specific sequence’s melting temperature can be used as a diagnostic for changes in the anticipated sequence makeup or solvent environment. In this webinar, we discuss how to use UV-visible absorption spectroscopy to determine the melting temperature of double-stranded DNA in different environments, including sequences containing base-pair mismatches.
Watch this webinar to learn about these topics:
- How to use UV-visible absorption techniques to determine the melting temperature of double-stranded DDBA
- Understanding the environmental factors that can change the calculated melting temperature of a DNA sample
About the presenter
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